Paula Cristina Branquinho de Andrade
Associate Professor
REQUIMTE/Laboratório de Farmacognosia, Departamento de Química, Faculdade de Farmácia, Universidade do Porto Rua de Jorge Viterbo Ferreira, nº. 228 4050-313 Porto Portugal
220 428 654
Research Interests
Metabolite profiling of natural matrices, marine and terrestrial, by several chromatographic techniques (LC-MS, HPLC-DAD, GC-MS).
Evaluation of bioactive agents from natural sources, comprising in vitro cellular and cell-free systems and in vivo models.
Current research is focused on marine organisms as source of bioactive agents, namely for cancer, assessed by the effect on cell viability, membrane integrity and cell density and mechanisms of cell death (necrosis or apoptosis).
Natural anti-inflammatory agents are also of interest. Evaluations include determination of L-citrulline, nitric oxide, superoxide radical and 3-nitrotyrosine levels, of pro-inflammatory cytokines (tumor necrosis factor-alpha (TNF-α), interleukin 1 and interleukin 6) and prostaglandins, their metabolites and isoprostanes.
Neurodegenerative diseases have been also addressed, by evaluating the effect on cholinesterases, muscarinic and nicotinic receptors and protection against neurocytotoxic agents.
Main publications
Pereira DM, Correia-da-Silva G, Valentão P, Teixeira N, ANDRADE PB. Anti-inflammatory effect of unsaturated fatty acids and ergosta-7,22-dien-3-ol from Marthasterias glacialis: prevention of CHOP-mediated ER-stress and NF-κB activation. PLoS ONE 2014, 9(2): e88341.

Pereira DM, Correia-da-Silva G, Valentão P, Teixeira N, ANDRADE PB. Palmitic acid and ergosta-7,22-dien-3-ol contribute to the apoptotic effect and cell cycle arrest of an extract from Marthasterias glacialis L. in neuroblastoma cells. Mar. Drugs 2014, 12, 54-68.

Pinho BR, Santos MM, Fonseca-Silva A, Valentão P, ANDRADE PB, Oliveira JMA. How mitochondrial dysfunction affects zebrafish development and cardiovascular function: An in vivo model for testing mitochondria-targeted drugs. Br. J. Pharmacol. 2013, 169, 1072-1090.

Moita E, Gil-Izquierdo A, Sousa C, Ferreres F, Silva LR, Valentão P, Domínguez-Perles R, Baenas N, ANDRADE PB. Integrated analysis of COX-2 and iNOS derived inflammatory mediators in LPS-stimulated RAW macrophages pre-exposed to Echium plantagineum L. bee pollen extract. PLoS ONE 2013, 8(3): e59131.

Pinho BR, Sousa C, Valentão P, ANDRADE PB. Is nitric oxide decrease observed with naphthoquinones in LPS stimulated RAW 264.7 macrophages a beneficial property? PLoS ONE 2011, 6 (8), e24098.