Biomodels, Bioanalytics and Biophysics

GROUP MEMBERS

Salette Reis (Associate Professor, Group Leader)
Eduarda Fernandes (Associate Professor)
Marcela Segundo (Assistant Professor)
Luís Magalhães (Assistant Researcher)
Renan Chisté (Assistant Researcher)
Sofia Lima (Assistant Researcher)
Cláudia Nunes (Post-doc)
Marisa Freitas (Post-doc)

 

LABs WEBSITEs

 

RESEARCH INTERESTS AND OBJECTIVES

The BBB group conveys expertise on physical-chemistry, medicinal chemistry, pharmaceutical and analytical sciences in order to support research towards biomedical challenges. The Group focuses on the study of the complex interactions of drugs, bioactive compounds and/or nanoparticles with lipid membranes and other biologically relevant structures. Several topics are pursued, including the interaction of nonsteroidal-anti-inflammatory, anti-cancer and anti-tuberculosis drugs with mimetic membrane models; the development of drug delivery systems for the treatment of infectious diseases, for dermocosmetic applications (cellulite and alopecia), and for administration of nutraceuticals (resveratrol).
Research activities are also focused on unrevealing the secrets of human neutrophils' oxidative burst, namely the role of these cells in the healing vs the deleterious effects of the inflammatory process. Other aspects related to oxidative stress are addressed, including the protective effects of carotenoids against the oxidative damage in human erythrocytes, the modulation of human neutrophils' oxidative burst by flavonoids and the potential anti-inflammatory effects of 2-styrylchromones.
Research towards novel bioanalytical techniques is another topic of interest, aiming the development of high-throughput and screening methods for bioactive compounds, namely those exerting antioxidant properties. Analytical methods based on molecular recognition, targeted to study biomembrane and inflammation phenomena, are also pursued.

 

REPRESENTATIVE PUBLICATIONS

Magalhães, L.M., Nunes, C., Lúcio, M., Segundo, M.A., Reis, S., Lima, J.L.F.C. (2010) High-throughput microplate assay for the determination of drug partition coefficients. NATURE PROTOCOLS 5(11), 1823-1830.

Lima, S.A.C., Resende, M., Silvestre, R., Tavares, J., Ouaissi, A., Lin, P.K.T., Cordeiro-da-Silva, A. (2012) Characterization and evaluation of BNIPDaoct-loaded PLGA nanoparticles for visceral leishmaniasis: in vitro and in vivo studies. NANOMEDICINE 7(12), 1839-1849.

Gomes, A., Fernandes, E., Silva, A.M.S., Pinto, D., Santos, C.M.M., Cavaleiro, J.A.S., Lima, J.L.F.C. (2009) Anti-inflammatory potential of 2-styrylchromones regarding their interference with arachidonic acid metabolic pathways. BIOCHEMICAL PHARMACOLOGY 78(2), 171-177.

Lúcio, M., Bringezu, F., Reis, S., Lima, J.L.F.C., Brezesinski, G. (2008) Binding of nonsteroidal anti-inflammatory drugs to DPPC: Structure and thermodynamic aspects. LANGMUIR 24(8), 4132-4139.

Nunes, C., Brezesinski, G., Pereira-Leite, C., Lima, J.L.F.C., Reis, S., Lúcio, M. (2011) NSAIDs Interactions with Membranes: A Biophysical Approach. LANGMUIR 27(17), 10847-10858.

Freitas, M., Fernandes, E. (2011) Zinc, cadmium and nickel increase the activation of NF-kappa B and the release of cytokines from THP-1 monocytic cells. METALLOMICS 3(11), 1238-1243.

Nunes, C., Brezesinski, G., Lima, J., Reis, S., Lúcio, M. (2011) Effects of non-steroidal anti-inflammatory drugs on the structure of lipid bilayers: therapeutical aspects. SOFT MATTER 7(6), 3002-3010.

Ribeiro, J.P.N., Magalhães, L.M., Segundo, M.A., Reis, S., Lima, J.L.F.C. (2010) Fully automatic flow method for the determination of scavenging capacity against nitric oxide radicals. ANALYTICAL AND BIOANALYTICAL CHEMISTRY 397(7), 3005-3014.

Estevão, M.S., Carvalho, L.C., Ribeiro, D., Couto, D., Freitas, M., Gomes, A., Ferreira, L.M., Fernandes, E., Marques, M.M.B. (2010) Antioxidant activity of unexplored indole derivatives: Synthesis and screening. EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY 45(11), 4869-4878.

Freitas, M., Porto, G., Lima, J.L.F.C., Fernandes, E. (2009) Optimization of experimental settings for the analysis of human neutrophils oxidative burst in vitro. TALANTA 78(4-5), 1476-1483.