Electron paramagnetic resonance (EPR) spectroscopy has been widely used to study the structure and function of different biochemical systems. Most of the studies have been focused on the redox centres of several metalloenzymes and other metalloproteins, on paramagnetic reaction intermediates and electron/radical transfer reactions, on the metabolism of biologically relevant radicals (e.g. nitric oxide and superoxide radicals) and on biological membranes fluidity. EPR has also been largely used to characterise non-biological systems, including (in)organic compounds, magnetic ionic liquids and membrane ageing and grafting mechanisms.
The EPR Facility is hosted by the Chemistry Department - FCT/UNL and is managed by the "Biological Chemistry @ FCT/UNL" group (http://sites.fct.unl.pt/biologicalchemistryatfctunl). It provides structural and functional spectroscopic information for researchers working at the Chemistry Department - FCT/UNL, as well as for several collaborators from other national and international research institutes.
The EPR Facility comprises an X-band spectrometer (Bruker EMX 6/1) and cavities to work at variable temperatures (4 to 295K), for perpendicular and parallel modes (Bruker rectangular cavities), with different types of samples. The variable low temperature is ensured by a continuous-flow cryostat (Oxford Instruments ESR900) working with liquid helium.
The EPR Facility provides a comprehensive service (service provider), assisted by trained PhD researchers, from sample preparation and logistics associated with the utilisation of the equipment to data analysis (identification, simulation, quantification) and assays interpretation/design/optimisation.