Alejandro Samhan-Arias
Post-doc Researcher
Integrated Member
Research Group
Structural Molecular Biology
212 948 300
- ext 10927
Research Interests
Research Activities - Keywords
• Metalloenzymes, flavoproteins, structure-activity relationships
• Reactive oxygen and nitrogen species (ROS/RNS) biochemistry
- formation/elimination and enzymes structure-activity relationships
• Mechanisms of ROS/RNS-mediated diseases
• Cytochrome b5 reductase (rat, pig and human), cytochrome b5, cytochrome c
• Plasma membrane NAD(P)H oxidases
• Reconstitution of electron transfer chains
• Molecular modeling (protein-protein interactions, protein-ligand interactions)
• Oxidative lipidomics
Main publications
• High expression of cytochrome b 5 reductase isoform 3/cytochrome b 5 system in the cerebellum and pyramidal neurons of adult rat brain.
Samhan-Arias AK, López-Sánchez C, Marques-da-Silva D, Lagoa R, Garcia-Lopez V, García-Martínez V, Gutierrez-Merino C
Brain Struct Funct [2015]

• Purified NADH-cytochrome b5 reductase is a novel superoxide anion source inhibited by apocynin: sensitivity to nitric oxide and peroxynitrite.
Samhan-Arias AK, Gutierrez-Merino C
Free Radic Biol Med [2014, 73:174-189]

• Lipidomics identifies cardiolipin oxidation as a mitochondrial target for redox therapy of brain injury.
Ji J, Kline AE, Amoscato A, Samhan-Arias AK, Sparvero LJ, Tyurin VA, Tyurina YY, Fink B, Manole MD, Puccio AM, Okonkwo DO, Cheng JP, Alexander H, Clark RS, Kochanek PM, Wipf P, Kagan VE, Bayır H
Nat Neurosci [2012, 15(10):1407-1413]
PMID:22922784 PMCID:PMC3697869

• Oxidized phospholipids as biomarkers of tissue and cell damage with a focus on cardiolipin.
Samhan-Arias AK, Ji J, Demidova OM, Sparvero LJ, Feng W, Tyurin V, Tyurina YY, Epperly MW, Shvedova AA, Greenberger JS, Bayır H, Kagan VE, Amoscato AA
Biochim Biophys Acta [2012, 1818(10):2413-2423]
PMID:22464971 PMCID:PMC3398793

• Stimulation and clustering of cytochrome b5 reductase in caveolin-rich lipid microdomains is an early event in oxidative stress-mediated apoptosis of cerebellar granule neurons.
Samhan-Arias AK, Marques-da-Silva D, Yanamala N, Gutierrez-Merino C
J Proteomics [2012, 75(10):2934-2949]

• A mitochondria-targeted inhibitor of cytochrome c peroxidase mitigates radiation-induced death.
Atkinson J, Kapralov AA, Yanamala N, Tyurina YY, Amoscato AA, Pearce L, Peterson J, Huang Z, Jiang J, Samhan-Arias AK, Maeda A, Feng W, Wasserloos K, Belikova NA, Tyurin VA, Wang H, Fletcher J, Wang Y, Vlasova II, Klein-Seetharaman J, ...
Nat Commun [2011, 2:497]
PMID:21988913 PMCID:PMC3557495