angelina.palma

Maria Angelina de Sá Palma
Assistant Researcher
Integrated Member
Research Group
Structural Molecular Biology
Gab. 6.34 Department of Chemistry FCT-UNL Caparica
212 948 300
- ext 10940
212 948 550
Research Interests
Carbohydrate-ligand discovery for recognition systems of biological, biomedical and biotechnological importance using carbohydrate microarrays; structural analysis of the protein-carbohydrate interaction by protein X-ray crystallography; neoglycolipid-based oligosaccharide microarray technology; ‘designer’ microarrays from microbial and plant polysaccharides (Glucans, LPS and others); glucan-binding proteins of the immune system e.g. Dectin-1; lectin-carbohydrate interaction in the immune system; mammalian and microbial carbohydrate binding proteins (e.g. lectins, CBMs and glycoside hydrolases, antibodies); host-pathogen interactions mediated by carbohydrate recognition; cancer-associated carbohydrate antigens; recombinant protein expression; protein crystallization; protein and protein-ligand structure determination.
Main publications
PUBLICATIONS IN REFERRED JOURNALS
- Neu, U., Allen, S.A., Blaum, B.S., Liu, Y., Frank, M., Palma, A.S., Ströh, L.J., Feizi, T., Peters T., Atwood, W.J., Stehle, T. “A structure-guided mutation in the major capsid protein retargets BK Polyomavirus”. PLoS Pathog 9:e1003688, 2013

- Neu, U., Mahmood Khan, Z., Schuch, B., Palma, A.S., Liu, Y., Pawlita, M., Feizi, T., Stehle, T. “Structures of B-Lymphotropic Polyomavirus VP1 in complex with oligosaccharide ligands”. PLoS Pathog 9:e1003714, 2013.

- Crusat, M1., Liu, J1., Palma, A.S1., Childs, R.A1., Liu, Y1., Wharton, S.A., Lin, Y.P., Coombs, P.J., Martin, S.R., Matrosovich, M., Chen, Z., Stevens, D.J., Hien, V.M., Thanh, T.T., Nhu, L.N., Nguyet, L.A., Ha, D.Q., van Doorn, H.R., Hien, T.T., Conradt, H.S., Kiso, M., Gamblin, S.J., Chai, W., Skehel, J.J., Hay, A.J., Farrar, J., de Jong, M.D., Feizi, T. Changes in the hemagglutinin of H5N1 viruses during human infection - Influence on receptor binding. Virology, 447: 326-337, 2013 (1co-first authors).

- Palma, A.S., Liu, Y., Childs, R.A., Herbert, C., Wang, D, Chai, W, Feizi, T. “The human epithelial carcinoma antigen recognized by monoclonal antibody AE3 is expressed on a sulfoglycolipid in addition to neoplastic mucins” Biochem Biophys Res Commun. 408:548-52, 2011

- Capodicasa, C., Chiani, P., Bromuro, C., De Bernardis, F., Catellani, M., Palma, A.S., Liu, Y., Feizi, T., Cassone, A., Benvenuto, E., Torosantucci, A. “Plant production of anti-β-glucan antibodies for immunotherapy of fungal infections in humans” Plant Biotechnol J 9:776-87, 2011

- Neu, U., Maginnis, M.S., Palma, A.S., Stroeh, L.J., Nelson, C.D., Feizi,T., Atwood, W.J., Stehle, T.. Structure-function analysis of the human JC polyomavirus establishes the LSTc pentasaccharide as a functional receptor motif. Cell Host & Microbe 8:309-19, 2010
Press release: http://news.brown.edu/pressreleases/2010/10/polyoma

- Liu, Y., Childs, R.A., Matrosovich, T., Wharton, S., Palma, A.S., Chai, W., Daniels, R., Gregory, V., Uhlendorff, J., Kiso, M., Klenk, H.D., Hay, A., Feizi, T., Matrosovich, M. Altered receptor specificity and cell tropism of D222G haemagglutinin mutants from fatal cases of pandemic A(H1N1) 2009 influenza. J Virol 84: 12069-74, 2010

- Palma, A.S1. Childs R.A1., Wharton S., Matrosovich T., Liu Y., Chai W., Campanero-Rhodes M.A., Zhang Y., Eickmann M., Kiso M., Hay A., Matrosovich M., Feizi T. “Receptor-binding specificity of pandemic influenza A (H1N1) 2009 virus determined by carbohydrate microarray” Nat Biotechnol. 27:797-9, 2009 (1co-first authors).
Press release: http://www3.imperial.ac.uk/newsandeventspggrp/imperialcollege/newssummary/news_10-9-2009-11-16-38

- Torosantucci, A., Chiani, P., Bromuro, C., De Bernardis, F., Palma, A.S., Liu, Y., Mignogna, G., Maras, B., Colone, M., Stringaro, A., Zamboni, S., Feizi, T., Cassone, A. “Protection by anti-β-Glucan Antibodies is associated with restricted β-1,3 glucan binding specificity and inhibition of fungal growth and adherence” PLoS ONE 4, e5392, 2009.

- Schallus, T., Jaeckh, C., Féeher, K., Palma, A.S., Liu, Y., Simpson, J.C., Mackeen, M., Stier, G., Gibson, T.J. Feizi, T., Pieler, T. and Muhle-Goll, C. “Malectin - a novel carbohydrate-binding protein of the endoplasmic reticulum and a new player in the early steps of protein N-glycosylation” Mol Biol Cell 19: 3404-3414, 2008.

- Palma, A.S., Feizi, T., Zhang, Y., Stoll, M.S., Lawson, A.M., Díaz-Rodríguez, E., Campanero-Rhodes, M.A., Costa, J., Gordon, S., Brown, G.D. and Chai, W. “Ligands for the beta-glucan receptor, dectin-1, assigned using ‘designer’ microarrays of oligosaccharide probes (neoglycolipids) generated from glucan polysaccharides”. J Biol Chem 281: 5771-5779, 2006.

REVIEWS
- Palma, A.S., Feizi, T., Childs, R.A, Chai, W. and Liu, Y. “The neoglycolipid (NGL)-based oligosaccharide microarray system poised to decipher the meta-glycome”. Curr Opin Chem Biol, 18, 87-94, 2014.

- Liu, Y., Palma, A.S., Feizi, T. “Carbohydrate microarrays: key developments in glycobiology” Biol Chem 390, 647-56, 2009

CHAPTERS IN BOOKS
- Palma, A.S*., Zhang, Y., Childs, R.A., Campanero-Rhodes, M.A., Liu, Y., Feizi, T., Chai, W. “Neoglycolipid-Based "Designer" Oligosaccharide Microarrays to Define ß-Glucan Ligands for Dectin-1”. Yann Chevolot (ed.), Carbohydrate Microarrays: Methods and Protocols, Methods in Molecular Biology, Volume 808, Chapter 23:337-59, 2012 (*Corresponding author).

- Liu, Y., Childs, R.A., Palma, A.S., Campanero-Rhodes, M.A., Stoll, M.S., Chai, W., Feizi, T. “Neoglycolipid-Based Oligosaccharide Microarray System: Preparation of NGLs and Their Noncovalent Immobilization on Nitrocellulose-Coated Glass Slides for Microarray Analyses”. Yann Chevolot (ed.), Carbohydrate Microarrays: Methods and Protocols, Methods in Molecular Biology, Volume 808, Chapter 8:117-36, 2012

- Palma, A.S., Liu, Y., Muhle-Goll, C., Butters, T.D., Zhang, Y., Childs, R., Chai, W., Feizi, T. "Multifaceted approaches including neoglycolipid oligosaccharide microarrays to ligand discovery for malectin". Methods in Enzymology, Elsevier Inc. Volume 478, Chapter 13: 265-86, 2010