b.Pinheiro

Benedita Pinheiro
Post-doc Researcher
Collaborator
Research Group
Structural Molecular Biology
Departamento de Química Faculdade de Ciências e Tecnologia Universidade Nova de Lisboa 2829-516 Caparica, Portugal
212 948 300
- ext 10962
212 948 550
Research Interests
Study in Protein Biochemistry. Employment of molecular biology techniques such as polymerase chain
reaction (PCR) and cloning into plasmid for protein expression. Employment of various techniques of
protein purification, including chromatography on immobilized metal affinity (IMAC), size exclusion
chromatography and polyacrylamide gel electrophoresis (native and denaturing). Application of techniques
of crystallography as protein crystallization techniques (suspending drop, sitting drop, crystal seeding),
crystal fishing and freezing, synchrotron data collection and analysis, model building and phase refinement.
Utilization of isothermal titration calorimetry for obtaining calorimetric parameters of protein-protein
binding. Investigation of proteins acting on carbohydrates and complex enzymatic degrading of plant cell
wall. Development of protein microarrays.
Main publications
Pinheiro, B. A., Proctor, M. R., Martinez-Fleitez, C., Prates, J. A. M., Money, V. A., Davies, G.J., Bayer, E. A., Fontes, C. M. G. A., Fierobe, H., Gilbert, H. J. (2008) The Clostridium cellulolyticum dockerin displays a dual binding mode for its cohesin partner. Journal of Biological Chemistry, VOL. 283, NO.26, pp. 18422-18430. Impact factor of 5.520.

• Najmudin, S., Pinheiro, B.A., Romão, M.J., Prates, J.A.M, Fontes, C.M.G.A. (2008) Purification, crystallization and cristallographic analysis of Clostridium thermocelum endo-1,4-6 b-D-xylanase 10B in complex with xylohexahose. Acta Crystallographica, F64, pp. 715-718. Impact factor of 0.606.

• Montanier C., Money V.A., Pires V.M., Flint J.E., Pinheiro B.A., Goyal A., Prates J.A., Izumi A., Stålbrand H., Morland C., Cartmell A., Kolenova K., Topakas E., Dodson E.J., Bolam D.N., Davies G.J., Fontes C.M., Gilbert H.J. (2009) The active site of a carbohydrate esterase displays divergent catalytic and noncatalytic binding functions. PLoS Biology, VOL. 7, Issue 3, e1000071. Impact factor of 12,683.

• Pinheiro BA, Gilbert HJ, Sakka K, Sakka K, Fernandes VO, Prates JA, Alves VD, Bolam DN, Ferreira LM, Fontes CM (2009) Functional insights into the role of novel type I cohesin and dockerin domains from Clostridium thermocellum. Biochemical Journal, VOL.424, NO.3, pp. 375-384. Impact factor of 4,371.

• Najmudim S, Pinheiro BA, Prates JAM, Gilbert HJ, Romão MJ, Fontes CMGA (2010) Putting an N-terminal end to the Clostridium thermocellum xylanase Xyn10B story: Crystal structure of the CBM22-1-GH10 modules complexed with xylohexaose. Journal of Structural Biology. Impact factor of 4,059.

• Pinheiro BA, Brás JLA, Najmudin S, Carvalho AL, Ferreira LMA, Prates JAM, Fontes CMGA (2012) Flexibility and specificity of the cohesin–dockerin interaction: implications for cellulosome assembly and functionality. Biocatalysis and Biotransformation, VOL. 30, No. 3, pp. 309-315. Impact factor 0.895.