c.coelho

Catarina Coelho
Post-doc Researcher
Integrated Member
Research Group
Structural Molecular Biology
Macromolecular Crystallography Laboratory Chemistry Department Faculty of Sciences and Technology, Universidade NOVA de Lisboa 2829-516 Caparica Portugal
212 948 300
- ext 10962
Research Interests
I am an enthusiastic scientific research professional with 11 years of experience in Academia. My main area of expertize is related with Structural Biology, namely Macromolecular Protein Crystallography (MX). In the past recent years I have conducted several projects aiming to solve the 3D structure of proteins with high impact in human health and biotechnological processes, particularly mammalian aldehyde oxidases (AOX) and bacterial nitrate reductases (Nap).

My short CV comprises a PhD (2011) in Structural Biology at REQUIMTE from Faculdade de Ciências e Tecnologia - Universidade Nova de Lisboa in collaboration with the Instituto Mario Negri (Italy) and the University of Potsdam (Germany) where I was a visiting research scientist for several periods of time. Since 2013 I hold a Post-doctoral research position at UCIBIO. During my research professional experience I have published 14 papers in peer reviewed scientific journals from which 7 as a first author and one in NATURE CHEMICAL BIOLOGY (Coelho et al, 2015).

We have solved the first crystal structure of a mammalian aldehyde oxidase - the mouse AOX3, and more recently the structure of the human enzyme in the substrate free form and in complex with the substrate phthlazine and the inhibitor thioridazine (hAOX). I am now very much interested in studying the molecular determinants responsible for clinical drugs interactions with AOX to better understand the role of the enzyme in the failure of several clinical trials Phase I. I am also studying the impact of several single nucleotide polimorphisms (SNP) described in hAOX and their altered structural features which have proven to cause impact in the metabolism of the different individuals.

During this years I have also gained experience in protein expression and purification and in biochemical characterization of macromolecules (mainly using thermal shift assays). I am also very much familiarized with the use of several different synchrotron radiation sources in Europe (European synchrotron radiation facility, ESRF - Grenoble, France; Diamond Light Source, DLS - Didcot, UK; and Swiss Light Source, SLS - Villigen, Switzerland) in which we have conducted several experiments for data collection, analysis and interpretation."
Main publications
"Structure and function of mammalian aldehyde oxidases"
Mineko Terao, Maria João Romão, Silke Leimkuhler, Marco Bolis, Maddalena Frateli, Catarina Coelho, Teresa Santos-Silva and Enrico Garattini.
ARCH TOXICOL; 90:753-780, 2016
DOI: 10.1007/s00204-016-1683-1

"Structural insights into xenobiotic and inhibitor binding to human aldehyde oxidase"
Catarina Coelho, Alessandro Foti, Tobias Hartmann, Teresa Santos-Silva, Silke Leimkuhler and Maria João Romão.
NATURE CHEM BIOL; 11(10):779-83, 2015
DOI: 10.1038/nchembio.1895

"Structural and mechanistic insights on nitrate reductaes"
Catarina Coelho and Maria João Romão
PROTEIN SCIENCE; 24:1901-1911, 2015
doi: 10.1002/pro.2801

"The First Mammalian Aldehyde Oxidase Crystal Structure INSIGHTS INTO SUBSTRATE SPECIFICITY"
Catarin Coelho, Martin Mahro, José Trincão, Alexandra Carvalho, Maria João Ramos, Mineko Terao, Enrico Garattini, Silke Leimkuhler and Maria João Romão.
Journal of Biological Chemistry; Vol 287, Pages: 40690-40702 Published: NOV 23 2012
DOI: 10.1074/jbc.M112.390419

"The Crystal Structure of Cupriavidus necator Nitrate Reductase in Oxidized and Partially Reduced States"
Catarina Coelho, Pablo González, José Moura, Isabel Moura, José Trincão and Maria João Romão.
Journal of Molecular Biology; Vol 408, Pages: 932-948 Published: MAY 20 2011
DOI: 10.1016/j.jmb.2011.03.016

"The use of ionic liquids as crystallization additives allowed to overcome nanodrop scaling up problems: A success case for producing diffraction-quality crystals of a nitrate reductase"
Catarina Coelho, José Trincão and Maria João Romão
Journal of Crystal Growth; Vol 312, Pages: 714-719 Published: FEB 15 2010
DOI: 10.1016/j.jcrysgro.2009.11.060