Carlos Alberto Gomes Salgueiro
Assistant Professor
Integrated Member
Research Group
Structural Molecular Biology
Requimte-CQFB, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Campus Caparica, 2829-516 Caparica, Portugal
212 948 300
- ext 10936
212 948 550
Research Interests
1. Structure-function studies in metalloproteins, which include i) thermodynamic characterization and ii)NMR solution protein structure determination.
2. Study of the electron transfer pathways of bacteria involved in removal of toxic and radionuclides metals
3.Optimization of metalloproteins for Bioremediation, Electricity production and Biofuels production using genetic engineering tools
Main publications
Morgado, L., Fernandes, A. P., Londer, Yuri Y., Pokkuluri, P.R., Schiffer, M., Salgueiro, C.A. (2009)Thermodynamic characterization of the redox centers in a representative domain of a novel c-type multiheme cytochrome. Biochem. J. 420, 485-492.

Morgado, L., Fernandes, A. P., Londer, Yuri Y., Bruix, M., Salgueiro, C.A. (2010) One simple step in the identification of the cofactors signals, one giant leap for the solution structure determination of multiheme proteins. Biochem. Biophys. Res. Commun. 393, 466–470.

Inoue K., Qian X., Morgado L., Kim B.C., Mester T., Izallalen M., Salgueiro C.A., Lovley D.R. (2010) Purification and characterization of OmcZ, an outer-surface, octaheme c-type cytochrome essential for optimal current production by Geobacter sulfurreducens. Appl Environ Microbiol. 76, 3999-4007.

Morgado L., Bruix M., Pessanha M., Londer Y.Y., Salgueiro C.A. (2010) Thermodynamic characterization of a triheme cytochrome family from Geobacter sulfurreducens reveals mechanistic and functional diversity. Biophys J. 99, 293-301.

Morgado L., Saraiva I.H., Louro R.O., Salgueiro C.A. (2010) Orientation of the axial ligands and magnetic properties of the hemes in the triheme ferricytochrome PpcA from G. sulfurreducens determined by paramagnetic NMR. FEBS Lett. 584, 3442-3445.

Catarino, T., Pessanha, M., De Candia, A.G., Gouveia, Z., Fernandes, A.P., Pokkuluri, P.R., Murgida, D., Marti, M.A., Todorovic, S., Salgueiro, C.A. (2010) Probing the chemotaxis periplasmic sensor domains from Geobacter sulfurreducens by combined Resonance Raman and Molecular Dynamic approaches: NO and CO sensing, J. Phys. Chem. B 114, 11251-11260.

Pokkuluri, P.R., Londer, Y.Y, Duke, N., Pessanha, M., Yang, X., Orshonsky, V., Orshonsky, L., Erickson, J., Zagyansky, Y., Salgueiro, C.A., Schiffer, M. (2011) Structure of a novel dodecaheme cytochrome c from Geobacter sulfurreducens reveals an extended 12 nm protein with interacting hemes, Journal Structural Biology.

X. Qian, T. Mester, L. Morgado, T. Arakawa, M.L. Sharma, K. Inoue, C. Joseph, C.A. Salgueiro, M.J. Maroney, D.R. Lovley, Biochemical characterization of purified OmcS, a c-type cytochrome required for insoluble Fe(III) reduction in Geobacter sulfurreducens, Biochim Biophys Acta. 1807 (2011) 404-412.

Dantas J.M., Morgado L., Londer Y.Y., Fernandes A.P., Louro R.O., Pokkuluri P.R., Schiffer M., Salgueiro C.A. (2012) Pivotal role of the strictly conserved aromatic residue F15 in the cytochrome c7 family. J. Biol. Inorg. Chem. 17, 11-24.

Dantas J.M., Saraiva I.H., Morgado L., Silva M.A., Schiffer M., Salgueiro C.A., Louro R.O.(2011) Orientation of the axial ligands and magnetic properties of the hemes in the cytochrome c(7) family from Geobacter sulfurreducens determined by paramagnetic NMR. Dalton Trans. 40, 12713-12718.

Morgado L, Paixão VB, Schiffer M, Pokkuluri PR, Bruix M, Salgueiro C.A. (2012) Revealing the structural origin of the redox-Bohr effect: the first solution structure of a cytochrome from Geobacter sulfurreducens. Biochem J. 441,179-187.

Morgado L, Dantas JM, Bruix M, Londer YY, Salgueiro CA (2012) Fine-tuning of redox networks on multiheme cytochromes from Geobacter sulfurreducens drives physiological electron/proton energy transduction, Bioinorg. Chem. Appl., 1-9.

Silva MA, Lucas TG, Salgueiro CA, Gomes CM (2012). Protein folding modulates the swapped dimerization mechanism of methyl-accepting chemotaxis heme sensors. PLoS One. 2012;7(9):e46328. doi: 10.1371/journal.pone.0046328.

Morgado L, Dantas JM, Simões T, Londer YY, Pokkuluri PR, Salgueiro CA (2012). Role of methionine 58 in the regulation of electron/proton transfer in trihaem cytochrome PpcA from Geobacter sulfurreducens. Biosci Rep. 2012 Oct 2.

Morgado L, Fernandes AP, Dantas JM, Silva MA, Salgueiro CA. (2012) On the road to improve the bioremediation and electricity-harvesting skills of Geobacter sulfurreducens: functional and structural characterization of multihaem cytochromes. Biochem Soc Trans. 1;40(6):1295-301.

Dantas JM, Morgado L, Pokkuluri PR, Turner DL, Salgueiro CA. (2013) Solution structure of a mutant of the triheme cytochrome PpcA from Geobacter sulfurreducens sheds light on the role of the conserved aromatic residue F15. Biochim Biophys Acta. doi:pii: S0005-2728(12)01108-5.10.1016/j.bbabio.2012.12.008.