george

Georgina Correia da Silva
Assistant Professor
Integrated Member
Research Group
Drugs Targets & Biomarkers
Faculdade Farmácia Universidade do Porto Lab Bioquímica, Dep. Ciências Biológicas 4050-313 Porto
220 428 558
Research Interests
Tissue remodeling, apoptosis and autophagy, cell differentiation.
Study of the biological activities of new molecules as enzyme inhibitors with pharmacological interest in breast and prostate cancers.Endocrine resistance.
Endocannabinoids as signalling molecules: Role of the endocannabinoid system in the fetal-placental unit.
Main publications
- Almada M, Costa L, Fonseca BM, Amaral C, Teixeira N, Correia-da-Silva G. The synthetic cannabinoid WIN-55,212 induced-apoptosis in cytotrophoblasts cells by a mechanism dependent on CB1 receptor. Toxicology. 2017 Jun 15;385:67-73. doi: 10.1016/j.tox.2017.04.013.

- Valente MJ, Amaral C, Correia-da-Silva G, Duarte JA, Bastos ML, Carvalho F, Guedes de Pinho P, Carvalho M. Methylone and MDPV activate autophagy in human dopaminergic SH-SY5Y cells: a new insight into the context of β-keto amphetamines-related neurotoxicity. Arch Toxicol. 2017 May 19. doi: 10.1007/s00204-017-1984-z.

- Fonseca BM, Teixeira NA, Correia-da-Silva G. Cannabinoids as Modulators of Cell Death: Clinical Applications and Future Directions. Rev Physiol Biochem Pharmacol. 2017;173:63-88. doi: 10.1007/112_2017_3.

- Amaral C, Varela CL, Maurício J, Sobral AF, Costa SC, Roleira FMF, Tavares-da-Silva EJ, Correia-da-Silva G, Teixeira N. Anti-tumor efficacy of new 7α-substituted androstanes as aromatase inhibitors in hormone-sensitive and resistant breast cancer cells. J Steroid Biochem Mol Biol. 2017 Jul;171:218-228. doi: 10.1016/j.jsbmb.2017.04.002.

- Costa MA, Fonseca BM, Mendes A, Braga J, Teixeira NA, Correia-da-Silva G. The endocannabinoid 2-arachidonoylglycerol dysregulates the synthesis of proteins by the human syncytiotrophoblast. Biochim Biophys Acta. 2016 Mar;1861(3):205-12. doi: 10.1016/j.bbalip.2015.12.008.

- Almada M, Fonseca BM, Amaral C, Diniz-da-Costa M, Correia-da-Silva G, Teixeira N. Anandamide oxidative metabolism-induced endoplasmic reticulum stress and apoptosis. Apoptosis. 2017 Feb 20. doi: 10.1007/s10495-017-1356-4.

- Almada M, Amaral C, Diniz-da-Costa M, Correia-da-Silva G, Teixeira NA, Fonseca BM.The endocannabinoid anandamide impairs in vitro decidualization of human cells. Reproduction. 2016 Oct;152(4):351-61. doi: 10.1530/REP-16-0364.

- Varela CL, Amaral C, Correia-da-Silva G, Costa SC, Carvalho RA, Costa G, Alcaro S, Teixeira NA, Tavares-da-Silva EJ, Roleira FM. Exploring new chemical functionalities to improve aromatase inhibition of steroids. Bioorg Med Chem. 2016 Jun 15;24(12):2823-31. doi: 10.1016/j.bmc.2016.04.056.

- Sobral AF, Amaral C, Correia-da-Silva G, Teixeira N. Unravelling exemestane: From biology to clinical prospects. J Steroid Biochem Mol Biol. 2016 Oct;163:1-11. doi: 10.1016/j.jsbmb.2016.03.019.

- Almada M, Cunha S, Fonseca BM, Amaral C, Piscitelli F, Di Marzo V, Correia-da-Silva G, Teixeira N. Anandamide interferes with human endometrial stromal-derived cell differentiation: An effect dependent on inhibition of cyclooxygenase-2 expression and prostaglandin E2 release. Biofactors. 2016 May;42(3):277-86. doi: 10.1002/biof.1270.

- Costa L, Amaral C, Teixeira N, Correia-da-Silva G, Fonseca BM. Cannabinoid-induced autophagy: Protective or death role? Prostaglandins Other Lipid Mediat. 2016 Jan;122:54-63. doi: 10.1016/j.prostaglandins.2015.12.006

- Amaral C, Lopes A, Varela CL, da Silva ET, Roleira FM, Correia-da-Silva G, Teixeira N. Exemestane metabolites suppress growth of estrogen receptor-positive breast cancer cells by inducing apoptosis and autophagy: A comparative study with Exemestane. Int J Biochem Cell Biol. 2015 Dec;69:183-95. doi: 10.1016/j.biocel.2015.10.024.

- Almada M, Piscitelli F, Fonseca BM, Di Marzo V, Correia-da-Silva G, Teixeira N. Anandamide and decidual remodelling: COX-2 oxidative metabolism as a key regulator. Biochim Biophys Acta. 2015 Nov;1851(11):1473-81. doi: 10.1016/j.bbalip.2015.08.011.

- Costa MA, Fonseca BM, Mendes A, Braga J, Teixeira NA, Correia da Silva G. The endocannabinoid anandamide affects the synthesis of human syncytiotrophoblast-related proteins. Cell Tissue Res. 2015 Nov;362(2):441-6. doi: 10.1007/s00441-015-2236-2.

- Costa MA, Fonseca BM, Marques F, Teixeira NA, Correia-da-Silva G The psychoactive compound of Cannabis sativa, Δ(9)-tetrahydrocannabinol (THC) inhibits the human trophoblast cell turnover. Toxicology. 2015 Aug 6;334:94-103. doi: 10.1016/j.tox.2015.06.005.

- Fonseca BM, Correia-da-Silva G, Teixeira NA. Anandamide restricts uterine stromal differentiation and is critical for complete decidualization. Mol Cell Endocrinol. 2015 Aug 15;411:167-76. doi: 10.1016/j.mce.2015.04.024.

- Pereira DM, Valentão P, Correia-da-Silva G, Teixeira N, Andrade PB. Translating endoplasmic reticulum biology into the clinic: a role for ER-targeted natural products? Nat Prod Rep. 2015 May;32(5):705-22. doi: 10.1039/c4np00102h.

- Almada M, Domingues MR, Dória ML, Fonseca BM, Teixeira NA, Correia-da-Silva G. Lipidomic approach towards deciphering anandamide effects in rat decidual cell. J Cell Physiol. 2015 Jul;230(7):1549-57. doi: 10.1002/jcp.24901.

- Costa MA, Fonseca BM, Teixeira NA, Correia-da-Silva G. The endocannabinoid anandamide induces apoptosis in cytotrophoblast cells: involvement of both mitochondrial and death receptor pathways. Placenta. 2015 Jan;36(1):69-76. doi: 10.1016/j.placenta.2014.10.011.

- Costa MA, Fonseca BM, Keating E, Teixeira NA, Correia-da-Silva G. Transient receptor potential vanilloid 1 is expressed in human cytotrophoblasts: induction of cell apoptosis and impairment of syncytialization. Int J Biochem Cell Biol. 2014 Dec;57:177-85. doi: 10.1016/j.biocel.2014.10.008.

- Varela CL, Amaral C, Tavares da Silva E, Lopes A, Correia-da-Silva G, Carvalho RA, Costa SC, Roleira FM, Teixeira N. Exemestane metabolites: Synthesis, stereochemical elucidation, biochemical activity and anti-proliferative effects in a hormone-dependent breast cancer cell line. Eur J Med Chem. 2014 Nov 24;87:336-45. doi: 10.1016/j.ejmech.2014.09.074.

- Costa MA, Keating E, Fonseca BM, Teixeira NA, Correia-da-Silva G. 2-Arachidonoylglycerol impairs human cytotrophoblast cells syncytialization: influence of endocannabinoid signalling in placental development. Mol Cell Endocrinol. 2015 Jan 5;399:386-94. doi: 10.1016/j.mce.2014.09.005.

- Fonseca BM, Battista N, Correia-da-Silva G, Rapino C, Maccarrone M, Teixeira NA. Activity of anandamide (AEA) metabolic enzymes in rat placental bed. Reprod Toxicol. 2014 Nov;49:74-7. doi: 10.1016/j.reprotox.2014.07.078.

- Fonseca BM, Almada M, Costa MA, Teixeira NA, Correia-da-Silva G. Rat spontaneous foetal resorption: altered α2-macroglobulin levels and uNK cell number. Histochem Cell Biol. 2014 Dec;142(6):693-701. doi: 10.1007/s00418-014-1252-8.

- Pereira DM, Correia-da-Silva G, Valentão P, Teixeira N, Andrade PB. Anti-inflammatory effect of unsaturated fatty acids and Ergosta-7,22-dien-3-ol from Marthasterias glacialis: prevention of CHOP-mediated ER-stress and NF-κB activation. PLoS One. 2014 Feb 13;9(2):e88341. doi: 10.1371/journal.pone.0088341.

- Pereira DM, Correia-da-Silva G, Valentão P, Teixeira N, Andrade PB. GC-MS lipidomic profiling of the echinoderm Marthasterias glacialis and screening for activity against human cancer and non-cancer cell lines. Comb Chem High Throughput Screen. 2014;17(5):450-7.

- Costa MA, Fonseca B, Keating E, Teixeira N, Correia-da-Silva G (2014) 2-arachidonoylglycerol effects in cytotrophoblasts: Metabolic enzymes expression and apoptosis in BeWo cells. Reproduction. 3;147(3):301-11.

- Pereira DM, Correia-da-Silva G, Valentão P, Teixeira N, Andrade PB. (2013) Palmitic Acid and Ergosta-7,22-dien-3-ol Contribute to the Apoptotic Effect and Cell Cycle Arrest of an Extract from Marthasterias glacialis L. in Neuroblastoma Cells. Mar Drugs. Dec 24;12(1):54-68. doi: 10.3390/md12010054.

- Fonseca B, Correia-da-Silva G, Teixeira N (2013) "The endocannabinoid anandamide induces apoptosis of rat decidual cells through a mechanism involving ceramide synthesis and p38 MAPK activation" Apoptosis 18(12) : 1526-35.

- Cristina Amaral, Carla Varela, Georgina Correia-da-Silva, Elisiário Tavares da Silva, Rui A. Carvalho, Saul C. P. Costa, Sara C. Cunha, José O. Fernandes, Natércia Teixeira, Fernanda M. F. Roleira (2013). “New Steroidal 17ß-Carboxy Derivatives Present Anti-5a-Reductase Activity and Anti-proliferative Effects in a Human Androgen-Responsive Prostate Cancer Cell Line.” Biochimie 95 (11): 2097-106.

- Amaral C, Varela C, Borges M, Tavares da Silva E, Roleira FM, Correia-da-Silva G, Teixeira N. (2013) Steroidal aromatase inhibitors inhibit growth of hormone-dependent breast cancer cells by inducing cell cycle arrest and apoptosis. Apoptosis Nov 18 (11) : 1426-36.

- Fonseca BM, Correia-da-Silva G, Almada M, Costa MA, Teixeira NA (2013) "The endocannabinoid system in the postimplantation period: A role during decidualization and placentation" Int J Endocrinol 2013;2013:510540. Epub 2013 Oct 21.

- Cristina Amaral,Sara C Cunha,José O Fernandes,Elisiário T Silva,Fernanda Roleira, Natércia Teixeira and Georgina Correia-da-Silva (2013) Development of a new Gas Chromatography - Mass Spectrometry (GC-MS) methodology for the evaluation of 5alpha-reductase activity" .Talanta, Mar 30;107:154-61.

- Cristina Amaral, Carla Varela, Margarida Azevedo, Elisiário Tavares da Silva, Fernanda Roleira, Shiuan Chen, Georgina Correia-da-Silva, Natércia Teixeira. (2013). Effects of steroidal aromatase inhibitors on sensitive and resistant breast cancer cells: aromatase inhibition and autophagy”, J Steroid Biochem Mol Biol. May, 135: 51-9.

- Fonseca BM, Costa MA, Almada M, Correia-da-Silva G, Teixeira NA. (2013) Endogenous cannabinoids revisited: a biochemistry perspective. Prostaglandins Other Lipid Mediat. Apr-May;102-103:13-30.

- Varela CL, Amaral C, Correia-da-Silva G, Carvalho RA, Teixeira NA, Costa SC, Roleira FM, Tavares-da-Silva EJ. Design, synthesis and biochemical studies of new 7a-allylandrostanes as aromatase inhibitors. Steroids. 2013 Jul;78(7):662-9.

- Amaral C, Borges M, Melo S, da Silva ET, Correia-da-Silva G, Teixeira N. (2012) Apoptosis and Autophagy in Breast Cancer Cells following Exemestane Treatment. PLoS One. 2012;7(8):e42398.

- Bruno M Fonseca, Georgina Correia-da-Silva, Anthony H Taylor, Patricia MW Lam, Timothy H Marczylo, Justin C Konje, Natércia A Teixeira (2012) Characterization of the endocannabinoid system in rat haemochorial placenta. Reprod Toxicol. 34(3):347-56

- Varela C, Tavares da Silva EJ, Amaral C, Correia da Silva G, Baptista T, Alcaro S, Costa G, Carvalho RA, Teixeira NA, Roleira FM (2012) New structure-activity relationships of A- and D-ring modified steroidal aromatase inhibitors: design, synthesis, and biochemical evaluation. J Med Chem. 26;55(8):3992-4002.

- Fonseca B.M., Correia-da-Silva G., Teixeira N.A. (2012) Rat as animal model during fetoplacental development: a reappraisal of post-implantation period. Reprod Biol 12(2): 97-118.

- Pereira DM, Valentao P, Correia-da-Silva G, Teixeira N, Andrade PB (2012). Plant secondary metabolites in cancer chemotherapy: where are we? Curr Pharm Biotechnol. Apr;13(5):632-50.

- Fonseca, B. M., N. A. Teixeira, M. Almada, A. H. Taylor, J. C. Konje and G. Correia-da-Silva (2011) Modulation of the novel cannabinoid receptor - GPR55 - during rat fetoplacental development. Placenta 32(6): 462-469.

- Pereira D, Vinholes J, Correia-da-Silva, Valentão P, Teixeira N, Andrade P (2011) Fatty acids in marine organisms: in the persuit of bioactive agents. Current Pharmaceutical Analysis 7, 108-119.

-B.M. Fonseca, G. Correia-da-Silva, A.H. Taylor, P.M.W. Lam, T.H. Marczylo, S.C. Bell, J.C. Konje and N.A. Teixeira (2010). The endocannabinoid 2-arachidonoylglycerol (2-AG) and metabolizing enzymes during rat fetoplacental development: A role in uterine remodelling. Int J Biochem Cell Biol. Nov;42(11):1884-92.

-Fonseca B.M., Correia-da-Silva G., Taylor A.H., Lam P.M.W., Marczylo T.H., Konje J.C., Bell S.C., Teixeira N.A. (2010) N-acylethanolamine levels and expression of their metabolizing enzymes during pregnancy. Endocrinology, 151(8):3965-74.

-Fonseca B.M., Correia-da-Silva G., Teixeira N.A. (2009) Anandamide-Induced Cell Death: Dual Effects in Primary Rat Decidual Cell Cultures. Placenta, 30(8): 686-692.

-Fonseca BM, Correia-da-Silva G, Taylor AH, Konje JC, Bell SC, Teixeira NA. (2009)
Spatio-temporal expression patterns of anandamide-binding receptors in rat implantation sites: evidence for a role of the endocannabinoid system during the period of placental development.
Reproductiove Biology Endocrinology 27(7):121-134.

-E Teixeira de Lemos, F Reis, S Baptista, R Pinto, B Sepodes, H Vala, P Rocha-Pereira, G Correia da Silva, N Teixeira, A Santos Silva, L Carvalho, F Teixeira, Das Un (2009) Exercise training decreases proinflammatory profile in Zucker Diabetic (Type 2) Fatty rats. Nutrition 25:330-39.

-Cepa M, Correia-da-Silva G, da Silva EJ, Roleira MF, Borges M, Teixeira NA (2008) New steroidal aromatase inhibitors: supression of estrogen-dependent breast cancer cell proliferation and induction of cell death. BMC Cell Biology 24(9):41-54.

-Cepa M, Correia-da-Silva G, Tavares da Silva EJ, Roleira FM, Hong Y, Chen S, Teixeira NA (2008) Molecular mechanisms of aromatase inhibition by new a, D-ring modified steroids. Biological Chemistry 389(9):1183-91.

-Cepa M, Tavares da Silva EJ, Correia-da-Silva G, Roleira MF, Teixeira NA (2008) Synthesis and biochemical studies of 17-substituted androst-3-enes and 3,4-Epoxyandrostanes as aromatase inhibitors. Steroids 73 (14):1409-15.

-Almeida A, Correia-da-Silva G, Cepa M, Bell SC, Teixeira NA (2007) Synergistic induction of apoptosis in primary rat decidual cells by INF-gamma and TNF. Molecular Reproduction and development 74(3):371-7.

-Cepa M, Tavares da Silva EJ, Correia-da-Silva G, Roleira MF, Teixeira NA (2005) Structure-activity relationships of new A, D-ring modified steroids as aromatase inhibitors: design, synthesis and biological activity evaluation. Journal Medicinal Chemistry 48 (20):6379-85.

-Correia-da-Silva G, Bell Sc, Pringle JH, Teixeira NA (2005) Patterns of expression of Bax, Bcl-2 and Bcl-x(L) in the implantation site in rat during pregnancy. Placenta 26(10):796-806.

-Correia-da-Silva G, Bell Sc, Pringle JH, Teixeira NA (2004) Patterns of uterine cellular proliferation and apoptosis in the implantation site of the rat during pregnancy. Placenta 25(6):538-47.

Abstracts in Conferences:

- Marta Almada, Bruno Fonseca, Miguel Morais, Felix Carvalho, Natércia Teixeira, Georgina Correia-da-Silva. The impact of phyto and synthetic cannabinoids in placental cytotrophoblast cells turnover. XIX National Congress of Biochemistry 2016, University of Minho, Guimarães; 8th to the 10th of December 2016.