Gilda de Sousa Carvalho Oehmen
Assistant Researcher
Integrated Member
Research Group
Design of Molecules and NanoMaterials for Therapy and Diagnosis
Lab. 505 Chemistry Department Faculty of Sciences and Technology New University of Lisbon 2829-516 Caparica
212 948 571
- ext 10991
Research Interests
Wastewater treatment and resource recovery using mixed microbial cultures
• Micropollutant mitigation (pharmaceuticals, pesticides, etc)
• Nutrient removal (phosphorus and nitrogen)
• Production of biopolymers (polyhydroxyalkanoates) from industrial wastes
• Production of platform substances (organic acids) from waste
• Microbial community assessment with molecular biology tools (fluorescence in situ hybridisation (FISH), high throughput sequencing, quantitative PCR, denaturing gradient gel electrophoresis (DGGE), amplicon cloning and sequencing).
Main publications
1. Castanheira N, Dourado AC, Kruz S, Alves PI, Delgado-Rodríguez AI, Pais I, Semedo J, Scotti-Campos P, Sánchez C, Borges N, Carvalho G, Barreto Crespo M, Fareleira P (2016). Plant growth promoting Burkholderia species isolated from annual ryegrass in Portuguese soils, Journal of Applied Microbiology. doi: 10.1111/jam.13025. IF: 2.479, Q2, CN: 0.
2. Franca RDG, Vieira A, Mata AM, Carvalho G, Pinheiro HM, Lourenço ND (2015). Effect of an azo dye on the performance of an aerobic granular sludge sequencing batch reactor treating a simulated textile wastewater, Water Research 85, 327-336 IF: 5.323, Q1, CN: 0.
3. Marques, R., Oehmen, A., Carvalho, G. and Reis, M.A.M. (2015). Modelling the Biodegradation Kinetics of the Herbicide Propanil and its Metabolite 3,4-Dichloroaniline, Environmental Science and Pollution Research 22: 6687-6695. DOI: 10.1007/s11356-014-3870-z. IF: 2.757, Q2, CN: 0
4. Diniz, M.S., Salgado R., Pereira, V.J., Oehmen A., Reis M.A.M., Noronha J.P. and Carvalho G. (2015). Ecotoxicity of ketoprofen, diclofenac and atenolol and their phololysis byproducts in zebrafish (Danio rerio), Science of the Total Environment, 505: 282-289. IF: 3.163, QI, CN: 0.
5. Carvalheira M, Oehmen A, Carvalho G, Reis MAM. (2014). Survival strategies of polyphosphate accumulating organisms and glycogen accumulating organisms under conditions of low organic loading. Bioresource Technology 172: 290-296. IF: 5.039, CN: 0, Q1.
6. Lanham AB, Oehmen A, Saunders AM, Carvalho G, Nielsen PH and Reis MAM. (2014). Metabolic modelling of full-scale enhanced biological phosphorus removal sludge: anaerobic TCA cycle vs. glycolysis, Water Research, 66: 283-295. IF: 5.323, Q1, CN: 2.
7. Carvalheira M, Oehmen A, Carvalho G, Eusebio M and Reis MAM. (2014). The impact of aeration on the competition between polyphosphate accumulating organisms and glycogen accumulating organisms, Water Research, 66: 296-307. IF: 5.323, Q1, CN: 2.
8. Carvalheira M, Oehmen A, Carvalho G and Reis MAM (2014). The effect of substrate competition on the metabolism of polyphosphate accumulating organisms (PAOs), Water Research, 64: 149-159. IF: 5.323, Q1, CN: 3.
9. Carvalho, G.*, Oehmen, A., Albuquerque, M.G.E. and Reis, M.A.M. (2014). The relationship between mixed microbial culture composition and PHA production performance from fermented molasses, New Biotechnology 31(4): 257-263. IF: 2.106, Q3, CN: 1.
10. Lanham AB, Oehmen A, Saunders AM, Carvalho G*, Nielsen PH and Reis MAM. (2013). Metabolic versatility in full-scale wastewater treatment plants performing enhanced biological phosphorus removal, Water Research, 47(9), 7032-7041. IF: 5.323, Q1, CN: 8.
11. Lanham AB, Ricardo AR, Albuquerque MGE, Pardelha F, Carvalheira M, Coma M, Fradinho J, Carvalho G, Oehmen A, Reis MAM (2013). Determination of the extraction kinetics for the quantification of polyhydroxyalkanoate monomers in mixed microbial systems. Process Biochemistry 48(11): 1626-1634. IF: 2.524,Q1, CN: 4.
12. Pardelha, F., Albuquerque, M.G.E., Carvalho, G., Reis, M.A.M., Dias, J.M.L. and Oliveira, R. (2013). Segregated flux balance analysis constrained by population structure/function data: the case of PHA production by mixed microbial cultures. Biotechnology and Bioengineering, 110(8): 2267-2276. IF: 4.164, Q1, CN: 2.
13. Fradinho, J., Domingos, J., Carvalho, G., Oehmen, A. and Reis, M.A.M. (2013). Polyhydroxyalkanoates production by a mixed photosynthetic consortium of bacteria and algae. Bioresource Technology 132, 146-153. IF: 5.039, Q1, CN: 4.
14. Almeida, B., Oehmen, A., Marques, R., Brito, D., Carvalho, G.*, Barreto Crespo, M.T. (2013). Modelling the biodegradation of non-steroidal anti-inflammatory drugs (NSAIDs) by activated sludge and a pure culture. Bioresource Technology, 133, 31-37. IF: 5.039, Q1, CN: 5.
15. Almeida, B., Vaz-Moreira, I., Schumann, P., Nunes, O.C., Carvalho, G., Barreto Crespo, M.T. (2013). Patulibacter medicamentivorans sp. nov., isolated from activated sludge of a wastewater treatment plant. International Journal of Systematic and Evolutionary Microbiology 63(7), 2588-2593. IF: 2.798, Q3, CN: 0.
16. Almeida, B., Kjeldal, H., Lolas, I., Knudsen, A. D., Carvalho, G., Nielsen, K.L., Barreto Crespo, M.T., Stensballe, A., and Nielsen, J.L. (2013). Quantitative proteomic analysis of ibuprofen-degrading Patulibacter sp. strain I11. Biodegradation 24(5), 615-630. IF: 2.492, Q2, CN: 6.
17. Salgado R., Pereira, V.J., Carvalho G.*, Soeiro R., Gaffney V., Almeida C., Vale Cardoso V., Ferreira E., Benoliel M.J., Ternes T.A., Oehmen A., Reis M.A.M. and Noronha J.P. (2013). Photodegradation kinetics and transformation products of ketoprofen, diclofenac and atenolol in pure water and treated wastewater. Journal of Hazardous Materials, 244-245, 516-527. IF: 4.331, Q1, CN: 20.
18. Oehmen A, Marques R, Noronha JP, Carvalho G, Reis MAM. (2013). Propionate addition enhances the biodegradation of the xenobiotic herbicide propanil and its metabolite. Bioresource Technology, 127, 195-201. IF: 5.039, Q1, CN: 3.
19. Albuquerque M.G.E., Carvalho G., Kragelund C., Silva A.F., Barreto Crespo M.T., Reis M.A.M. and Nielsen P.H. (2013). Link between microbial composition and carbon substrate uptake preferences in a PHA-storing mixed culture. ISME Journal, 7, 1-12. IF: 9.267, Q1, CN: 14.
20. Galinha C, Guglielmi G, Carvalho G, Portugal CM, Crespo JG, Reis MAM. (2013). Development of a hybrid model strategy for monitoring membrane bioreactors. Journal of Biotechnology, 164 (2013), 386-395. IF: 2.884, Q2, CN: 1.
21. Salgado R., Oehmen A., Carvalho G., Noronha J.P. and Reis M.A.M. (2012). Biodegradation of clofibric acid and identification of its metabolites. Journal of Hazardous Materials, 241-242, 182:189. IF: 4.331, Q1, CN: 6.
22. Ricardo A.R., Carvalho G., Velizarov S., Crespo J.G., Reis M.A.M. (2012). Kinetics of nitrate and perchlorate removal and biofilm stratification in an ion exchange membrane bioreactor. Water Research, 46(14), 4556-4568, IF: 5.323, Q1, CN: 15.
23. Lanham AB, Ricardo AR, Coma M, Fradinho J, Carvalheira M, Oehmen A, Carvalho G and Reis MAM. (2012). Optimisation of glycogen quantification in mixed microbial cultures. Bioresource Technology, 118, 518:525. IF: 5.039, Q1, CN: 11.
24. Silva A.F., Carvalho G., Soares R., Coelho A.V., Barreto Crespo M.T. (2012). Step-by-step strategy for protein enrichment and proteome characterisation of extracellular polymeric substances in wastewater treatment systems. Applied Microbiology and Biotechnology, 95(3), 767-776. IF: 3.811, Q1, CN: 4.
25. Galinha C, Carvalho G*, Portugal CM, Guglielmi G, Reis MAM and Crespo JG. (2012). Multivariate statistically-based modelling of a membrane bioreactor for wastewater treatment using 2D fluorescence monitoring data. Water Research, 46(11), 3623-3636. IF: 5.323, Q1, CN: 11.
26. Acevedo B, Oehmen A, Carvalho G, Seco A, Borrás L and Barat R. (2012). Metabolic shift of polyphosphate-accumulating organisms with different levels of polyphosphate storage. Water Research, 46(6), 1889-1900. IF: 5.323, Q1, CN: 30.
27. Salgado R, Marques R, Noronha JP, Carvalho G, Oehmen A, Reis MAM. (2012). Assessing the Removal of Pharmaceuticals and Personal Care Products in a Full-Scale Activated Sludge Plant. Environmental Science and Pollution Research, 19(5) 1818-1827. IF: 2.757, Q2, CN: 29.
28. Silva, A.F., Carvalho, G.*, Oehmen, A., Lousada-Ferreira, M., van Nieuwenhuijzen, A., Reis, M.A.M. and Crespo, M.T.B. (2012). Microbial Population Analysis of Nutrient Removal-Related Organisms in Membrane Bioreactors. Applied Microbiology and Biotechnology, 93:2171-2180. IF: 3.811, Q1, CN: 19.
29. Salgado, R., Marques, R., Noronha, J.P., Oehmen, A., Carvalho, G. and Reis, M.A.M. (2011). Assessing the diurnal variability of pharmaceutical and personal care products in a full-scale activated sludge plant, Environmental Pollution, 159(10): 2359-2367. IF: 3.902, Q1, CN: 17.
30. Carvalho, G.*, Almeida, B., Oehmen, A., Fradinho, J., Reis, M.A.M. and Crespo, M.T.B. (2011). Microbial characterisation of mercury reducing mixed cultures enriched with different carbon sources, Microbes and Environments, 26(4), 293-300. IF: 2.424, Q2, CN: 2.
31. Galinha, C., Carvalho, G., Portugal, C.A.M., Guglielmi, G., Oliveira, R., Crespo, J.G. and Reis, M.A.M. (2011). Real-time monitoring of membrane bioreactors with 2D-fluorescence data and statistically-based models, Water Science and Technology, 63(7), 1381-1388. IF: 1.212, Q3, CN: 9.
32. Galinha, C., Carvalho, G., Portugal, C., Reis, M.A.M. and Crespo, J.G. (2011). Two-Dimensional Fluorescence as a Fingerprinting Tool for Monitoring Wastewater Treatment Systems. Journal of Chemical Technology and Biotechnology, 86(7), 985-992. IF: 2.494, Q1, CN: 5.
33. Salgado, R., Noronha, J.P., Oehmen, A., Carvalho, G.* and Reis, M.A.M. (2010), Analysis of 65 pharmaceuticals and personal care products in 5 wastewater treatment plants in Portugal using a simplified analytical methodology. Water Science and Technology, 62(12), 2862-2871. IF: 1.212, Q3, CN: 19.
34. Carvalho, G., Marques, R., Lopes, A.R., Faria, C., Noronha, J.P., Oehmen, A., Nunes, O.C. and Reis, M.A.M. (2010), Biological treatment of propanil and 3,4-dichloroaniline: kinetic and microbiological characterisation. Water Research, 44(17), 4980-4991. IF: 5.323, Q1, CN: 12.
35. Pereira, V. J., Fernandes, D., Carvalho, G., Benoliel, M. J., San Romão, M. V. and Barreto Crespo, M. T. (2010), Assessment of the presence and dynamics of fungi in drinking water sources using cultural and molecular methods. Water Research, 44(17), 4850-4859. IF: 5.323, Q1, CN: 24.
36. Oehmen, A., Carvalho, G., Lopez-Vazquez, C.M., van Loosdrecht, M.C.M. and Reis, M.A.M. (2010) Incorporating microbial ecology into the metabolic modelling of polyphosphate accumulating organisms and glycogen accumulating organisms. Water Research, 44(17), 4992-5004. IF: 5.323, Q1, CN: 41.
37. Oehmen A., Lopez-Vaquez C.M., Carvalho G., Reis M.A.M., van Loosdrecht M.C.M. (2010) Modelling the population dynamics and metabolic diversity of organisms relevant in anaerobic/anoxic/aerobic enhanced biological phosphorus removal processes. Water Research, 44(15), 4473-4486. IF: 5.323, Q1, CN: 33.
38. Oehmen, A., Carvalho, G., Freitas, F., Reis, M.A.M. (2010), Assessing the abundance and activity of denitrifying polyphosphate accumulating organisms through molecular and chemical techniques. Water Science and Technology, 61(8), 2061-2068. IF: 1.212, Q3, CN: 20.
39. Freitas, F., Temudo, M., Carvalho, G., Oehmen, A. and Reis, M.A.M. (2009), Robustness of sludge enriched with short SBR cycles for biological nutrient removal. Bioresource Technology, 100, 1969-1976. IF: 5.039, Q1, CN: 16.
40. Oehmen A., Fradinho J., Serra S., Carvalho G., Capelo J.L., Velizarov S., Crespo J.G., Reis M.A.M. (2009). The effect of carbon source on the biological reduction of ionic mercury. Journal of Hazardous Materials, 165(1-3), 1040-1048. IF: 4.331, Q1, CN: 23.
41. Carvalho, G., Lemos, P.C., Oehmen, A. and Reis, M.A.M. (2007), Denitrifying phosphorus removal: linking the process performance with the microbial community structure. Water Research, 41(19), 4383-4396. IF: 5.323, Q1, CN: 159.
42. Oehmen, A., Lemos, P. C., Carvalho, G., Yuan, Z., Keller, J., Blackall, L.L. and Reis, M.A.M. (2007), Advances in enhanced biological phosphorus removal: from micro to macro scale. Water Research, 41 (11), 2271-2300. IF: 5.323, Q1, CN: 380.
43. Ginige, M.P., Carvalho, G., Keller, J., Blackall, L.L. (2007). Eco-physiological characterization of fluorescence in situ hybridization probe-targeted denitrifiers in activated sludge using culture-independent methods. Letters in Applied Microbiology, 44 (4), 399-405. IF: 1.749, Q3, CN: 4.
44. Carvalho, G., Meyer, R.L., Yuan, Z., Keller, J. (2006). Differential distribution of ammonia- and nitrite-oxidising bacteria in flocs and granules from a nitrifying/denitrifying sequencing batch reactor. Enzyme and Microbial Technology, 39 (7), 1392-1398. IF: 2.966, Q2, CN: 17.
45. Carvalho, G., Novais, J.M., Pinheiro, H.M. and Vanrolleghem, P.A. (2004). Model development and application for surfactant biodegradation in an acclimatising activated sludge system. Chemosphere, 54, 1495-1502. IF: 3.499, Q1, CN: 11.
46. Carvalho, G., Novais, J.M. and Pinheiro, H.M. (2003). Activated sludge acclimatisation kinetics to non-ionic surfactants. Environmental Technology, 24 (1), 109-114. IF: 1.197, Q3, CN: 7.
47. Carvalho, G., Delee, W., Novais, J.M. and Pinheiro, H.M. (2002). A factorially-designed study of physico-chemical reactive dye colour removal from simulated cotton textile processing wastewaters. Coloration Technology, 118 (5), 215-219. IF: 1.173, Q3, CN: 9.
48. Carvalho, G., Novais, J.M., Vanrolleghem, P.A., Pinheiro, H.M. (2002). Optimal operation for timely adaptation of activated sludge plants to changes in the surfactant composition of wastewater. Water Science and Technology, 45 (4-5), 345-353. IF: 1.212, Q3, CN: 6.
49. Carvalho, G., Nopens, I., Novais, J.M., Vanrolleghem, P.A., Pinheiro, H.M. (2001). Modelling of activated sludge acclimatisation to a non-ionic surfactant. Water Science and Technology, 43 (7), 9-17. IF: 1.212, Q3, CN: 18.
50. Carvalho, G., Paul, E., Novais, J.M., Pinheiro, H.M. (2000). Studies on activated sludge response to variations in the composition of a synthetic surfactant-containing feed effluent. Water Science and Technology, 42 (5-6), 135-143. IF: 1.212, Q3, CN: 10.
51. Gouveia AR, Freitas EB, Galinha CF, Carvalho G, Duque AF and Reis MAM. Dynamic change of pH in acidogenic fermentation of cheese whey towards polyhydroxyalkanoates production: impact on performance and microbial population (submitted to New Biotechnology)
52. Oliveira CSS, Silva C, Carvalho G and Reis MAM. Strategies for efficiently selecting PHA producing mixed microbial cultures using complex feedstocks: internal and external selective pressures (submitted to New Biotechnology)
53. Silva AF, Antunes S, Saunders A, Freitas F, Vieira A, Nielsen PH, Barreto-Crespo MT, and Carvalho G Microbial population and extracellular polymeric substances diversity in membrane bioreactor under different sludge retention times (submitted to applied Microbiology and Biotechnology)
54. Wang X, Oehmen A, Freitas E, Carvalho G and Reis MAM. The impact of DO (dissolved oxygen) on substrate competition and PHA production performance in mixed microbial cultures (submitted to Water Research)
55. Silva AF, Antunes S, Carvalho G*, Reis MAM and Barreto Crespo MT. Role of extracellular polymeric substances in membrane fouling determined through membrane autopsy (in preparation)
56. Carvalho G*, Pedras I, Karst SM, Oliveira CSS, Duque AF, Nielsen PH and Reis MAM, Microbial community shift and performance with transition of feedstock in a 3-stage PHA-producing mixed culture system (in preparation).

(IF: impact factor; Q: journal quartile in category; CN: citations number; *: corresponding author)