hmsantos

Hugo Miguel Baptista Carreira dos Santos
Post-doc Researcher
Integrated Member
Research Group
Chem4Omics
BIOSCOPE Research Group | C4O | UCIBIO, Chemistry Department, Faculty of Science and Technology, University NOVA of Lisbon, Caparica Campus, Portugal
- ext 11405
Research Interests
Further developments of the application of oxygen 18 labelling of biological tissues for mass spectrometry imaging (MSI), including direct protein quantification on a tissue surface using MSI and isotopic labelling;
Characterization of oncogenic epidermal transformation in the Presenilin-deficient mice using proteomics analysis;
Application of MALDI-MSI for the identification of differentially expressed peptides and proteins of the transformation of normal epidermis to epidermal hyperplasia and finally to cancer;
Characterization of the peritoneal dialysis fluid’s proteome using LC-MALDI tandem mass spectrometry;
Personalised medicine in Autosomal Dominant Polycystic Kidney Disease (ADPKD);
Peptidomics and Proteomics;
Mass Spectrometry analysis of organic molecules including the interaction of organic molecules with metals and proteins.
Main publications
From a total of 40 peer-reviewed papers, 10 selected documents are presented bellow (Dec. 2013):

10. A comprehensive factorial design study of variables affecting protein extraction from formalin-fixed kidney tissue samples
Talanta 2014; 119:90-97. Doi:10.1016/j.talanta.2013.10.019.
IF. 3.498 (2012).
http://www.sciencedirect.com/science/article/pii/S0039914013008187

09. On-target ultrasonic digestion of proteins.
Proteomics. 2013 May;13(9):1423-7. doi: 10.1002/pmic.201200241.
IF. 4.132 (2012).
http://www.ncbi.nlm.nih.gov/pubmed/23457053

08. Fast human serum profiling through chemical depletion coupled to gold-nanoparticle-assisted protein separation.
Talanta. 2012 Oct 15;100:239-45. doi: 10.1016/j.talanta.2012.08.020.
IF. 3.498 (2012).
http://www.ncbi.nlm.nih.gov/pubmed/23141332

07. Probe-based chemical modulations of tissues for IMS.
J Proteomics. 2012 Aug 30;75(16):4921-30. doi: 10.1016/j.jprot.2012.05.025.
IF. 4.088 (2012).
http://www.ncbi.nlm.nih.gov/pubmed/22634403

06. Isotopic labelling of peptides in tissues enhances mass spectrometric profiling.
Rapid Commun Mass Spectrom.
2012 Feb 15;26(3):254-62. doi: 10.1002/rcm.5325.
IF. 2.509 (2012).
http://www.ncbi.nlm.nih.gov/pubmed/22223310

05. A comparison of depletion versus equalization for reducing high-abundance proteins in human serum.
Electrophoresis. 2011 Nov;32(21):2966-74. doi: 10.1002/elps.201100183.
IF. 3.303 (2011).
http://www.ncbi.nlm.nih.gov/pubmed/21997478

04. An assessment of the ultrasonic probe-based enhancement of protein cleavage with immobilized trypsin.
Proteomics. 2011 Oct;11(19):3866-76. doi: 10.1002/pmic.201100200.
IF. 4.505 (2011).
http://www.ncbi.nlm.nih.gov/pubmed/21805637

03. Decision peptide-driven: a free software tool for accurate protein quantification using gel electrophoresis and matrix assisted laser desorption ionization time of flight mass spectrometry.
Talanta. 2010 Sep 15;82(4):1412-20. doi: 10.1016/j.talanta.2010.07.007.
IF. 3.722 (2010).
http://www.ncbi.nlm.nih.gov/pubmed/20801349

02. A novel (18)O inverse labeling-based workflow for accurate bottom-up mass spectrometry quantification of proteins separated by gel electrophoresis.
Electrophoresis. 2010 Oct;31(20):3407-19. doi: 10.1002/elps.201000251.
IF. 3.569 (2010).
http://www.ncbi.nlm.nih.gov/pubmed/20882554

01. Light and colour as analytical detection tools: a journey into the periodic table using polyamines to bio-inspired systems as chemosensors.
Chem Soc Rev. 2010 Aug;39(8):2948-76. doi: 10.1039/b819787n.
IF. 26.585 (2010).
http://www.ncbi.nlm.nih.gov/pubmed/20548989