Ilda Santos Sanches
Associate Professor
Integrated Member
Research Group
Molecular Microbiology
Departamento de Ciências da Vida. Faculdade de Ciências e Tecnologia. Universidade Nova de Lisboa. Quinta da Torre. 2829-516 Caparica. Portugal
212 948 530
- ext 11113
212 948 530
Research Interests
The main research interest of our laboratory is to understand the dynamics of populations of bacterial pathogens from human and animal origin and the molecular mechanisms involved in pathogen adaptation and virulence.

Research activities include: - Comparison of phenotypes/genotypes of bacterial species/strains from different niches, clinical origins and hosts; - Analysis of the genomic structure and potential for horizontal gene transfer of phages (bacterial virus) and of other mobile genetic element; - Study of antimicrobial resistance patterns and mechanisms of resistance; - Investigation of the biological activity of new materials and new biocompounds against various pathogens; - Investigation of tailor-made strategies for infection control and risk assessment for bacterial colonization and infection.

Research Team

Ilda Santos Sanches, PhD, Principal Investigator

Rosario Mato, PhD,
Invited Assistant Professor, DCV-FCT/UNL

Nateson Balashubramanian, PhD, Postdoc fellow
(co-supervision A. Fernandes, DCV-FCT/UNL)

Catarina Rodrigues, PhD, Postdoc fellow
(co-supervision A. Fernandes, DCV-FCT/UNL)

Cinthia Barroco, PhD student
(co-supervision A. Fernandes, DCV-FCT/UNL)

Carlos Florindo, PhD student
(co-supervision M.J. Borrego, INSA. Portugal; B. Spellerberg, Ulm Univ. Germany)

Célia Leão PhD student
(co-supervision J. Inácio, University of Brighton, UK)

Tiago Dias, MSc, Graduate fellow
(co-supervision S. Gaudêncio, REQUIMTE-FCT/UNL)

João Caço, MSc student
(co-supervision A. Fernandes, DCV-FCT/UNL)

João Brito, MSc student
(co-supervision R. Mato, DCV-FCT/UNL)

Renato Pires, PhD (collaborator)

Márcia Rato, PhD (collaborator)
Main publications
Book Chapters

Santos Sanches, I., L. Chambel, and R. Tenreiro. 2015. Pulsed-Field Gel Electrophoresis (PFGE): Application in Population Structure Studies of Bovine Mastitis-Causing Streptococci, p. 323-334 (Ch. 23), In M. Cunha and J. Inácio (ed.), Veterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies, Methods in Molecular Biology, vol. 1247. Springer Science+Business Media New York , United States. ISBN 978-1-4939-2004-4. doi: 10.1007/978-1-4939-2004-4_23

Santos Sanches, I., and R. Bexiga. 2014. Epidemiologia molecular de Streptococcus uberis associados a mastites em bovinos: análise do polimorfismo de macrofragmentos de restrição separados por electroforese de campo pulsado (PFGE) e sequenciação de múltiplos loci (MLST), p. 307-313 (Ch. 26), In M. Cunha and J. Inácio (ed.), Abordagens Moleculares em Veterinária - Como desvendar a etiologia e a epidemiologia da infeção. Section IV: Exemplos práticos de aplicações moleculares em veterinária. Lidel Edições Técnicas Lda, Portugal. ISBN 978-989-752-034-1

Santos Sanches, I. 2014. Streptococcus, p. 255-276 (Ch. 21), In H. Barroso, A. Meliço-Silvestre, N. Taveira (ed.), Microbiologia Médica, Vol 1. Part IV. Bacteriologia médica. Lidel Edições Técnicas Lda, Portugal. ISBN 978-989-752-057-0


Almeida, A., C. Alves-Barroco, E. Sauvage, R. Bexiga, P. Albuquerque, F. Tavares, I. Santos-Sanches, and P. Glaser. 2016. Persistence of a dominant bovine lineage of group B Streptococcus reveals genomic signatures of host adaptation. Environ. Microbiol. In press.

- Prieto-Davo, A., T. Dias, S. E. Gomes, Y. Parera-Valadez, P. Borralho, F. Pereira, C. M. Rodrigues, I. Santos Sanches, and S. P. Gaudencio. 2016. The Madeira Archipelago as a significant source of marine-derived actinomycete diversity with anticancer and antimicrobial potential. Front. Microbiol. 7:1594. doi: 10.3389/fmicb.2016.01594

- Roma-Rodrigues, C., C. Alves-Barroco, L. R. Raposo, M. N. Costa, E. Fortunato, P. Viana Baptista, A. R. Fernandes, and I. Santos-Sanches. 2016. Infection of human keratinocytes by Streptococcus dysgalactiae subspecies dysgalactiae isolated from milk of the bovine udder. Microbes Infect. 18:290-293. doi: 10.1016/j.micinf.2015.11.005

- Vasilyeva, A., I. Santos Sanches, C. Florindo, and A. Dmitriev. 2015. Natural Mutations in Streptococcus agalactiae Resulting in Abrogation of β Antigen Production. PLoS One. 5;10(6):e0128426. doi: 10.1371/journal.pone.0128426

- Florindo C., V. Damião, I. Silvestre, C. Farinha, F. Rodrigues, F. Nogueira, F. Martins-Pereira, R. Castro, M. J. Borrego, I. Santos-Sanches, and The Group for the Prevention of Neonatal GBS Infection. 2014. Epidemiological surveillance of colonising group B Streptococcus epidemiology in the Lisbon and Tagus Valley regions, Portugal (2005 to 2012): emergence of a new epidemic type IV/clonal complex 17 clone. Euro Surveill. 2014;19(23):pii=20825.

- Leão C., D. Machado, I. Santos Sanches, I. Couto, M. Viveiros, J. Inácio, and A. Botelho. 2014. Relatedness of Mycobacterium avium subspecies hominissuis clinical isolates of human and porcine origins assessed by MLVA. Vet. Microbiol. 173: 92-100. doi: 10.1016/j.vetmic.2014.06.027

- Rato M. G., R. Bexiga, C. Florindo, L. M. Cavaco, C. L. Vilela, and I. Santos-Sanches. 2013. Antimicrobial resistance and molecular epidemiology of streptococci from bovine mastitis. Vet. Microbiol. 161: 286-294. doi: 10.1016/j.vetmic.2012.07.043

- Pires R., C. D. Rolo, A. Morais, A. Brito-Avô, C. Johansson, B. Henriques-Normark, J.Gonçalo-Marques, and I. Santos-Sanches. 2011. Description of macrolide-resistant and potential virulent clones of Streptococcus pyogenes causing asymptomatic colonization during 2000-2006 in Lisbon area. Eur. J. Clin. Microbiol. Infect. Dis. 31:849-857. doi: 10.1007/s10096-011-1384-x

- Florindo C., J. P. Gomes, M. G. Rato, L. Bernardino, B. Spellerberg, I. Santos-Sanches, and M. J. Borrego. 2011. Molecular epidemiology of group B streptococcal meningitis in children beyond the neonatal period from Angola. J. Med. Microbiol. 60: 1276-1280. doi: 10.1099/jmm.0.031674-0

- Rato M. G., A. Nerlich, R. Bergmann, R. Bexiga, S. F. Nunes, C. L. Vilela, I. Santos-Sanches and G. S. Chhatwal. 2011. Virulence gene pool detected in bovine group C Streptococcus dysgalactiae subsp. dysgalactiae using a group A Streptococcus pyogenes virulence microarray. J. Clin. Microbiol. 49:2470-2479. doi: 10.1128/JCM.00008-11

- Pires R., C. Ardanuy, D. Rolo, A. Morais, A. Brito-Avô, J.Gonçalo-Marques, J. Liñares, and I. Santos-Sanches. 2010. Emergence of ciprofloxacin-nonsusceptible Streptococcus pyogenes isolates from healthy children and pediatric patients in Portugal. Antimicrob. Agents Chemother. 54:2677-2780. doi: 10.1128/AAC.01536-09

- Pires R. D. Rolo, R. Mato, J. F. Almeida, C. Johansson. B. Henriques-Normark, A. Morais, A. Brito-Avô, J. G. Marques, and I. Santos-Sanches. 2009. Resistance to bacitracin in Streptococcus pyogenes from oropharyngeal colonization and noninvasive infections in Portugal was caused by two clones of distinct virulence genotypes. FEMS Microbiol. Lett. 296:235-240. doi: 10.1111/j.1574-6968.2009.01642.x

- Mato R., F. Almeida, R. Pires, P. Rodrigues, S. Carvalho, T. Ferreira, and I. Santos-Sanches. 2009. Assessment of high-level gentamicin and glycopeptide-resistant Enterococcus faecalis and E. faecium clonal structure in a Portuguese hospital over a 3-year period. Eur. J. Clin. Microbiol. Infect. Dis. 28:855-859. doi: 10.1007/s10096-009-0704-x