isn

Isabel de Sá-Nogueira
Associate Professor
Integrated Member
Research Group
Molecular Microbiology
Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa Departamento de Ciências da Vida Campus de Caparica, 2829-516 Caparica, Portugal
212 948 530
- ext 11104
212 948 530
Research Interests
Molecular Microbiology and Microbial Genetics. Control gene expression of carbohydrate metabolism in bacteria.
The main area of interest is to understand how the transcriptional and translational regulatory networks interact with other cellular components such as the metabolic system. This involves the analysis of the mechanisms through which the cell senses nutrient availability and transmits that information to the level of gene expression. It also involves looking at the transcription and translation control, which govern the expression of genes involved in carbohydrate metabolism in the Gram-positive model organism Bacillus subtilis. Another subject of interest is the use of B. subtilis as model for the study of these mechanisms in pathogenic bacteria.
Main publications
Recent publications:

Correia IL, Franco IS, de Sá-Nogueira I. (2014) Towards Novel Amino Acid-Base Contacts in Gene Regulatory Proteins: AraR - A Case Study. PLoS One. 9(11):e111802. doi: 10.1371/journal.pone.0111802.

McVey, C, Ferreira, M, Correia, B, Lahiri, S, de Sanctis, D, Carrondo, M A, Lindley, P, Sá-Nogueira, I, Soares, C, Bento, I (2014). The importance of the Abn2 calcium cluster in the endo-1,5-arabinanase activity from Bacillus subtilis Journal Biological Inorganic Chemistry 19(4-5):505-13. doi: 10.1007/s00775-014-1105-x.

Santos, R, Rocha, A, Matias, A, Duarte, C, Sá-Nogueira, I, Lourenço, N, Borges, JP and Vidinha P (2013) Development of Antimicrobial Ion Jelly fibers. RSC Advances 3, Issue 46, 24400-24405. doi: 10.1039/C3RA44258F.

Fontes, CMGA, Correia, I Sá, Romão, MJ, Sá-Nogueira, I, Prates, JAM, Ferreira, LMA (2012) Editorial of the 9th Carbohydrate Bioengineering Meeting (CBM9) Biocatalysis and Biotransformation, Vol. 30, No. 3, Pages 273-273. doi:10.3109/10242422.2012.681858.

Godinho LM, and de Sá-Nogueira I. (2011). Characterization and regulation of a bacterial sugar phosphatase of the HAD superfamily AraL from Bacillus subtilis. FEBS Journal 278: 2511-2524. doi: 10.1111/j.1742-4658.2011.08177.x

Ferreira M.J. and I. de Sá-Nogueira (2010) A multitask ATPase serving different ABC-type sugar importers in Bacillus subtilis. Journal of Bacteriology 192 (20): 5312-5318. doi: 10.1128/JB.00832-10

• de Sanctis, D., Inácio, J., Lindley, P., de-Sá-Nogueira, I. Bento, I. (2010). New evidence for the role of calcium in the glycosidase reaction of GH43 arabinanases. FEBS Journal 277(21): 4562-4574. doi: 10.1111/j.1742-4658.2010.07870.x

• Inácio, JM, Correia, I.L. and de Sá-Nogueira I. (2008). Two distinct arabinofuranosidases contribute to arabino-oligosaccharide degradation in Bacillus subtilis. Microbiology-SGM 154 (9): 2719-2729. doi: 10.1099/mic.0.2008/018978-0

de Sanctis D, Bento I, Inácio JM, Custódio S, de Sá-Nogueira I, and Carrondo MA (2008). Overproduction, crystallization and preliminary X-ray characterization of Abn2, an endo-1,5-alpha-arabinanase from Bacillus subtilis. Acta Crystallogr Sect F Struct Biol Cryst Commun. 64(Pt 7):636-638. doi:10.1107/S1744309108016321

Inácio, J.M., and I. de Sá-Nogueira. (2008). Characterization of the abn2(yxiA) encoding a Bacillus subtilis GH43 arabinanase, Abn2, and its role in arabino-polysaccharides degradation. Journal of Bacteriology 190: 4272-4280. doi:10.1128/JB.00162-08

Inácio J. M. and de Sá-Nogueira I. (2007) trans-acting factors and cis elements involved in glucose repression of arabinan degradation in Bacillus subtilis. Journal of Bacteriology 189(22): 8371-8376. doi:10.1128/JB.01217-07.

Franco. I.S. Mota. L.J. Soares. C.M. and de Sá-Nogueira I. (2007) Probing key DNA contacts in AraR-mediated transcriptional repression of the Bacillus subtilis arabinose regulon. Nucleic Acids Research 35: 4755–4766. Open Access. doi:10.1093/nar/gkm509.

Franco, S. I., Mota, L. J., Soares, C. M., and I. Sá-Nogueira. (2006). Functional domains of Bacillus subtilis transcription factor AraR and identification of aminoacids important for nucleoprotein complex assembly and effector-binding. Journal of Bacteriology 188: 3024-3036. doi:10.1128/JB.188.8.3024-3036.2006