msegundo

Marcela Alves Segundo
Assistant Professor
Integrated Member
Research Group
Biomodels, Bioanalytics and Biophysics
Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, R. Jorge Viterbo Ferreira, 228, 4050-313, Porto, Portugal
220 428 676
226 093 483
Research Interests

- liquid chromatography for determination of low weight molecules, nanoparticles and biomolecules;

- sample preparation using solid-phase extraction, dialysis and gas diffusion in batch or flow format.

- miniaturization and downscaling of wet chemistry methods;

- development of field deployable screening test.
Main publications
M. Rosende, L. M. Magalhães, M. A. Segundo, M. Miró, Automated microdialysis-based system for in-situ microsampling and investigation of lead bioavailability in terrestrial environments under physiologically-based extraction conditions, Environmental Science and Technology, 47, 11668–11675 (2013). doi: 10.1021/es401872j.

H. M. Oliveira, M. A. Segundo, A. J. M. Fonseca, A. R. J. Cabrita, Combining ultrasound assisted extraction and a microliter colorimetric assay for the streamlined and environmentally friendly determination of urea in animal feedstuff, Journal of Agricultural and Food Chemistry, 61, 9602–9608 (2013). doi: 10.1021/jf403037y.

C. M. Castro, M. Pinheiro, M. Lúcio, J. J. Giner-Casares, L. Camacho, J. L. F. C. Lima, S. Reis, M. A. Segundo, Insights about α-tocopherol and Trolox interaction with phosphatidylcholine monolayers under peroxidation conditions through Brewster Angle Microscopy, Colloids and Surfaces B: Biointerfaces, 111, 626–635 (2013). doi: 10.1016/j.colsurfb.2013.06.043.

L. M. Magalhães, L. Barreiros, M. A. Maia, S. Reis, J. L. F. C. Lima, M. A. Segundo*, Rapid assessment of endpoint antioxidant capacity of red wines through microchemical methods using a kinetic matching approach, Talanta, 97, 473-483 (2012). doi: 10.1016/j.talanta.2012.05.002.

S. M. Oliveira, H. M. Oliveira, M. A. Segundo, A. O. S. S. Rangel, J. L. F. C. Lima, V. Cerdà, Automated solid-phase spectrophotometric system for optosensing of bromate in drinking waters, Analytical Methods, 4, 1229-1236 (2012). doi: 10.1039/C2AY05860J.

L. M. Magalhães, M. I. G. S. Almeida, L. Barreiros, M. A. Segundo, S. Reis, Automatic aluminum chloride method for routine estimation of total flavonoids in red wines and teas, Food Analytical Methods, 5, 530-539 (2012). doi: 10.1007/s12161-011-9278-1.

H. M. Oliveira, M. A. Segundo*, J. L. F. C. Lima, Automatic flow system for evaluation of polystyrene-divinylbenzene sorbents applied to preconcentration of phenolic pollutants, International Journal of Environmental and Analytical Chemistry, 91, 884-899 (2011). doi: 10.1080/03067310903276316.

W. Boonjob, M. Miró, M. A. Segundo, V. Cerdà, Flow-through carbon nanofiber-based microsolid-phase extraction coupled to liquid chromatography for automatic determination of trace levels of priority herbicides in environmental matrices, Analytical Chemistry, 83, 5237-5244 (2011). doi: 10.1021/ac2005058.

M. I. G. S. Almeida, J. M. Estela, M. A. Segundo, V. Cerdà, A membraneless gas-diffusion unit - multisyringe flow injection spectrophotometric method for ammonium determination in untreated environmental samples, Talanta, 84, 1244-1252 (2011). doi: 10.1016/j.talanta.2010.12.052.

M. Rosende, M. Miró, M. A. Segundo, J. L. F. C. Lima, V. Cerdà, Highly integrated flow assembly for automated dynamic extraction and determination of readily bioaccessible chromium(VI) in soils exploiting carbon nanostructure-based solid-phase extraction, Analytical and Bioanalytical Chemistry, 400, 2217-2227 (2011). doi: 10.1007/s00216-011-4954-y.

J. P. N. Ribeiro, L. M. Magalhães, M. A. Segundo*, S. Reis, J. L. F. C. Lima, CUPRAC-FIA system for automatic determination of total antioxidant capacity in biological samples, Analytical Sciences, 27, 483-488 (2011). http://www.jstage.jst.go.jp/article/analsci/27/5/483/_pdf.

H. M. Oliveira, M. Miró, M. A. Segundo*, J. L. F. C. Lima, Universal approach for mesofluidic handling of bead suspensions in lab-on-valve format, Talanta, 84, 846-852 (2011). doi: 10.1016/j.talanta.2011.02.011.

C. I. C. Silvestre, P. C. A. G. Pinto, M. A. Segundo, M. L. M. F. S. Saraiva, J. L. F. C. Lima, Sequential injection enzyme based assays: a review, Analytica Chimica Acta, 689, 160-177 (2011). doi: 10.1016/j.aca.2011.01.048.

S. M. Oliveira, M. A. Segundo*, A. O. S. S. Rangel, J. L. F. C. Lima, V. Cerdà, Spectrophotometric determination of bromate in water using multisyringe flow injection analysis, Analytical Letters, 44, 284-297 (2011). doi: 10.1080/00032719.2010.500771.

M. Miró, H. M. Oliveira, M. A. Segundo, Analytical potential of the mesofluidic Lab-on-a-Valve platform as a front-end to column separation systems, Trends in Analytical Chemistry, 30, 153-164 (2011). doi: 10.1016/j.trac.2010.08.007

M. Iranifam, M. A. Segundo, J. L. M. Santos, José, L. F. C. Lima, M. H. Sorouraddin, Oscillating chemiluminescence systems: state of the art, Luminescence, 25, 409-418 (2010). doi: 10.1002/bio.1203

L. M. Magalhães, F. Santos, M. A. Segundo, S. Reis, J. L. F. C. Lima, Rapid microplate high-throughput methodology for assessment of Folin-Ciocalteu reducing capacity, Talanta, 83, 441-447 (2010). doi: 10.1016/talanta.2010.09.042

L. M. Magalhães, C. Nunes, M. Lúcio, M. A. Segundo, S. Reis, J. L. F. C. Lima, High-throughput microplate assay for the determination of drug partition coefficients, Nature Protocols, 5, 1823-1830 (2010). doi: 10.1038/nprot.2010.137

J. P. N. Ribeiro, L. M. Magalhães, M. A. Segundo*, S. Reis, J. L. F. C. Lima, Fully automatic flow method for the determination of scavenging capacity against nitric oxide radical, Analytical and Bioanalytical Chemistry, 397, 3005-3014 (2010). doi: 10.1007/s00216-010-3883-5

J. P. N. Ribeiro, L. M. Magalhães, M. A. Segundo*, S. Reis, J. L. F. C. Lima, Hydrogen peroxide, antioxidant compounds and biological targets: an in vitro scavenging approach using a MSFIA-fluorimetric system, Talanta, 81, 1840-1846 (2010). doi: 10.1016/j.talanta.2010.03.049

H. M. Oliveira, M. A. Segundo*, J. L. F. C. Lima, M. Miró, V. Cerdà, On-line renewable solid phase extraction hyphenated to liquid chromatography for the determination of UV filters using bead injection and multisyringe-lab-on-valve approach, Journal of Chromatography A, 1217, 3575-3582 (2010). doi: 10.1016/j.chroma.2010.03.035

W. Bonjoob, Y. Yu, M. Miró, M. A. Segundo, J. Wang, V. Cerdà, Online hyphenation of multimodal microsolid phase extraction involving renewable molecularly imprinted and reversed-phase sorbents to liquid chromatography for automatic multiresidue assays, Analytical Chemistry, 82, 3052-3050 (2010). doi: 10.1021/ac100185s

H. M. Oliveira, M. A. Segundo*, J. L. F. C. Lima, M. Miró, V. Cerdà, Exploiting automatic on-line renewable molecularly-imprinted solid-phase extraction in lab-on-valve format as front end to liquid chromatography: application to the analysis of riboflavin in foodstuffs, Analytical and Bioanalytical Chemistry, 397, 77-86 (2010). doi: 10.1007/s00216-010-3522-1