Structural Molecular Biology

GROUP MEMBERS

Isabel Moura (Full Professor)
José J. G. Moura (Full Professor)
Maria João Romão (Full Professor) (Group Leader)
Alice Santos Pereira (Assistant Professor)
Carlos Salgueiro (Assistant Professor)
Eurico Cabrita (Assistant Professor)
Pedro Tavares (Assistant Professor)
Sofia Rocha Pauleta  (Principal Researcher)
Ana Luísa Moreira de Carvalho (Assistant Researcher)
Maria Angelina de Sá Palma (Assistant Researcher)
Teresa Sacadura Santos Silva (Assistant Researcher)
Filipa Marcelo (Post-doc)

LABs WEBSITEs

Group Researcher ID 
http://www.researcherid.com/rid/M-5694-2013

 

RESEARCH INTERESTS AND OBJECTIVES

The team gathers complementary expertise ranging from protein expression and purification to biochemical, structural and functional characterization. There is extensive experience in X-ray Crystallography, NMR, EPR, Moessbauer, Glycan Microarrays, Fast Kinetics, (Bio)Electrochemistry among other biophysical techniques. The group has access to all necessary infrastructures for Structural Biology either in-house or within the scope of National or International Networks (PT NMR Network, ESRF and other SR labs (X-ray and SAXS)).
Researchers of the SMB Group have a long-standing interest in a variety of topics that can be grouped in two broad areas: environment impact and health & disease.
In several joint projects, the different methodologies available provide a complementary Systems Biology view and characterization of different biomolecular research topics: Protein structures, spectroscopic, biophysical and biochemical characterization; Enzyme structures and catalytic mechanisms; Structural and functional analysis of protein-protein, protein-ligand and protein-glycan interactions. These topics range from bioremediation and oxidative stress to drug action and design and are of central importance for a successful achievement of the strategic goals defined for all four Thematic Lines.

 

REPRESENTATIVE PUBLICATIONS

A. Viegas, J. Sardinha, F. Freire, A.L. Carvalho, C.M.G.A. Fontes, M.J. Romao, A.L. Macedo, E.J. Cabrita, Solution structure, dynamics and binding studies of a family 11 carbohydrate-binding module from clostridium thermocellum (CtCBM11), BIOCHEM J, 2013, 451(2), 289-300.

A.M. Figueiredo, J. Sardinha, G. R. Moore, E. J. Cabrita, Protein destabilisation in ionic liquids: The role of preferential interactions in denaturation, PHYS. CHEM. CHEM. PHYS., 2013, 15(45) 19632-19643.

Pereira, AS; Timoteo, CG; Guilherme, M; Folgosa, F; Naik , SG; Duarte, AG; Huynh, BH; Tavares, P; Spectroscopic Evidence for and Characterization of a Trinuclear Ferroxidase Center in Bacterial Ferritin from Desulfovibrio vulgaris Hildenborough, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 134; 26; 10822-10832 (2012). 

Dell'Acqua, S; Pauleta, SR; Moura, JJG; Moura, I; Biochemical characterization of the purple form of Marinobacter hydrocarbonoclasticus nitrous  oxide reductase, PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES,  Volume: 367; Issue:1593; Pages: 1204-1212 (2012). 

Timóteo CG, Guilherme M, Penas D, Folgosa F, Tavares P, Pereira AS. Desulfovibrio vulgaris bacterioferritin uses H(2)O(2) as a co-substrate for iron oxidation and reveals DPS-like DNA protection and binding activities., BIOCHEM J. 2012 Aug 15;446(1):125-33. doi: 10.1042/BJ20111439.

Morgado, L; Paixão, VB; Schiffer, M; Pokkuluri, PR; Bruix, M; Salgueiro, CA; Revealing the structural origin of the redox-Bohr effect: the first solution structure of a cytochrome from Geobacter sulfurreducens, BIOCHEM J., 441; 179-187 (2012). 

Santos-Silva, T; Mukhopadhyay, A; Seixas, JD; Bernardes, GJL; Romao, CC; Romao, MJ; CORM-3 Reactivity toward Proteins: The Crystal Structure of a Ru(II) Dicarbonyl-Lysozyme Complex, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,  133, 1192–1195 (2011). 

Coelho, C; Gonzalez, PJ; Moura, JJG; Moura, I; Trincao, J; Romao, MJ; The Crystal Structure of Cupriavidus necator Nitrate Reductase in Oxidized and Partially Reduced States, JOURNAL OF MOLECULAR BIOLOGY, 408/5 (2011). 

Santos-Silva, T; Ferroni, F; Thapper, A; Marangon, J; Gonzalez, PJ; Rizzi, AC; Moura, I; Moura, JJG; Romao, MJ; Brondino, CD; Kinetic, Structural, and EPR Studies Reveal That Aldehyde Oxidoreductase from Desulfovibrio gigas Does Not Need a Sulfido Ligand for Catalysis and Give Evidence for a Direct Mo-C Interaction in a Biological System, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 131/23 (2009). 

Childs, RA*; Palma, AS*; Wharton, S; Matrosovich, T; Liu, Y; Chai, W; Campanero-Rhodes, MA; Zhang, Y; Eickmann, M; Kiso, M; Hay, A; Matrosovich, M; Feizi, T; Receptor-binding specificity of pandemic influenza A (H1N1) 2009 virus determined by carbohydrate microarray, NATURE BIOTECHNOL, 27:797-9 (2009). (* co-first authors)

Marcelo, F; He, Y; Yuzwa, SA; Nieto, L; Jiménez-Barbero, J; Sollogoub, M; Vocadlo, DJ; Davies, GD; Blériot, Y; Molecular Basis for Inhibition of GH84 Glycoside Hydrolases by Substituted Azepanes; Conformational Flexibility Enables Probing of Substrate Distortion, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 131; 5390-5392 (2009).