OMAR BOUTUREIRA, Universitat Rovira i Virgili, Tarragona, Spain
Seminar: Chemical tools for selective protein modification
Host: Cecília Roque, UCIBIO
Site-selective chemical protein modification has emerged as a powerful strategy to install biophysical probes and post-translational modifications as well as for the conjugation of therapeutic payloads at pre-determined sites. A number of methodologies based on reactions that address biocompatibility, reactivity and (chemo)selectivity issues have been developed. Here we present selected examples of site-selective protein modification protocols including (a) the preparation of fluoroglycoproteins and (b) the development of Se-based bioconjugation methods as well as recent advances exploring small handles for (c) the chemoselective installation of secondary amine linkages on proteins via formation of C–N bonds at dehydroalanine-tagged proteins, (d) inverse electron demand Diels-Alder reactions (IEDDA), and (e) Ru(II)-hydrosilylation, among others.
Omar Boutureira is a Ramón y Cajal fellow at the Universitat Rovira i Virgili (URV), Tarragona, Spain. After receiving his B.Sc. in Chemistry and Ph.D. degree at the URV, he undertook postdoctoral research at the University of Oxford, UK and the University of Cambridge, UK. His research interests focus on various aspects of Chemical Biology and Glycobiology including chemical protein modification (with a particular emphasis on the development of targeted therapeutics/imaging probes for cancer diagnosis and treatment) and the use of halogens & chalcogens as well as metal-mediated strategies in glycoconjugate synthesis and carbohydrate chemistry.