The protein crystallization facility is dedicated to the production of single crystals of proteins (free or in complex with other proteins or ligands of interest), using conventional and/or automated methods (protein crystallization robot Oryx8, for sitting drop and microbatch screening and optimization). Crystals can be produced under anaerobic conditions. The 20ºC and 4ºC crystallization rooms permit incubation and storage of crystallization setups. Protein crystals are preserved and stored under cryogenic conditions.
The X-ray Diffraction facility provides the screening, testing and complete data collection from X-ray diffraction of single crystals, either from protein or small molecule compounds. Data is obtained in the in-house X-ray diffractometer or through access to synchrotron macromolecular crystallography beamlines (ESRF, DIAMOND, SLS, SOLEIL, DESY, ALBA). Dedicated software permits indexing, integration, scaling of data and 3D structure solution. The facility is equipped with a Bruker D8 Venture dual source X-ray generator (copper and molybdenum microfocus X-ray sources), a Photon 100 CMOS detector, and an Oxford cryo-system. A microspectrophotometer is available and can be coupled to the goniometer.
A service for Single Crystal X-ray Structure Determination of small molecules is available.
X-ray diffraction service through DQ-FCT-NOVA