Maria Paula do Amaral Alegria Guedes de Pinho

Independent Researcher

Research Group
Coordinating Investigator (since 2016)
Personal Website
Phone number
220428000 (8796)
Researcher ID

Main publications

  • Rodrigues D; Pinto J; Araújo AM; Monteiro-Reis S; Jerónimo C; Henrique R; Bastos ML; Guedes de Pinho P; Carvalho M. 2018. Volatile metabolomic signature of bladder cancer cell lines based on gas chromatography-mass spectrometry. Metabolomics, 14(62), DOI: 10.1007/s11306-018-1361-9
  • Lima AR; Araújo AM; Pinto J; Jerónimo C; Henrique R; Bastos ML; Carvalho M; Guedes de Pinho P. 2018. GC-MS-Based Endometabolome Analysis Differentiates Prostate Cancer from Normal Prostate Cells.. Metabolites, 8(1), DOI: 10.3390/metabo8010023
  • Lima, AR; Araujo, AM; Pinto, J; Jeronimo, C; Henrique, R; Bastos, MD; Carvalho, M; de Pinho, PG. 2018. Discrimination between the human prostate normal and cancer cell exometabolome by GC-MS. Scientific Reports, 8, DOI: 10.1038/s41598-018-23847-9
  • Araujo, AM; Carvalho, M; Carvalho, F; Bastos, MD; de Pinho, PG. 2017. Metabolomic approaches in the discovery of potential urinary biomarkers of drug-induced liver injury (DILI). CRITICAL REVIEWS IN TOXICOLOGY, 47, DOI: 10.1080/10408444.2017.1309638
  • Rodrigues, D; Monteiro, M; Jeronimo, C; Henrique, R; Belo, L; Bastos, MD; de Pinho, PG; Carvalho, M. 2017. Renal cell carcinoma: a critical analysis of metabolomic biomarkers emerging from current model systems. Translational Research, 180, DOI: 10.1016/j.trsl.2016.07.018
  • Monteiro, M; Moreira, N; Pinto, J; Pires-Luis, AS; Henrique, R; Jeronimo, C; Bastos, MD; Gil, AM; Carvalho, M; de Pinho, PG. 2017. GC-MS metabolomics-based approach for the identification of a potential VOC-biomarker panel in the urine of renal cell carcinoma patients. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 21, DOI: 10.1111/jcmm.13132
  • Calejo, I; Moreira, N; Araujo, AM; Carvalho, M; Bastos, MD; de Pinho, PG. 2016. Optimisation and validation of a HS-SPME-GC-IT/MS method for analysis of carbonyl volatile compounds as biomarkers in human urine: Application in a pilot study to discriminate individuals with smoking habits. TALANTA, 148, DOI: 10.1016/j.talanta.2015.09.070
  • Lima, AR; Bastos, MD; Carvalho, M; de Pinho, PG. 2016. Biomarker Discovery in Human Prostate Cancer: an Update in Metabolomics Studies. Translational Oncology, 9, DOI: 10.1016/j.tranon.2016.05.004
  • Monteiro, MS; Barros, AS; Pinto, J; Carvalho, M; Pires-Luis, AS; Henrique, R; Jeronimo, C; Bastos, MDL; Gil, AM; de Pinho, PG. 2016. Nuclear Magnetic Resonance metabolomics reveals an excretory metabolic signature of renal cell carcinoma. Scientific Reports, 6, DOI: 10.1038/srep37275
  • Rodrigues, D; Jeronimo, C; Henrique, R; Belo, L; Bastos, MD; de Pinho, PG; Carvalho, M. 2016. Biomarkers in bladder cancer: A metabolomic approach using in vitro and ex vivo model systems. INTERNATIONAL JOURNAL OF CANCER, 139, DOI: 10.1002/ijc.30016
  • Gil, AM; de Pinho, PG; Monteiro, MS; Duarte, IF. 2015. NMR metabolomics of renal cancer: an overview. Bioanalysis, 7, DOI: 10.4155/bio.15.167
  • Araujo, AM; Valente, MJ; Carvalho, M; da Silva, DD; Gaspar, H; Carvalho, F; Bastos, MD; de Pinho, PG. 2015. Raising awareness of new psychoactive substances: chemical analysis and in vitro toxicity screening of 'legal high' packages containing synthetic cathinones. ARCHIVES OF TOXICOLOGY, 89, DOI: 10.1007/s00204-014-1278-7
  • Silva, A; Sousa, E; Palmeira, A; Amorim, P; de Pinho, PG; Ferreira, DA. 2014. Interaction between hydroxyethyl starch and propofol: computational and laboratorial study. JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS, 32, DOI: 10.1080/07391102.2013.840248
  • Carvalho, LM; Carvalho, F; Bastos, MD; Baptista, P; Moreira, N; Monforte, AR; Ferreira, ACD; de Pinho, PG. 2014. Non-targeted and targeted analysis of wild toxic and edible mushrooms using gas chromatography-ion trap mass spectrometry. TALANTA, 118, DOI: 10.1016/j.talanta.2013.09.038
  • Monteiro, M; Carvalho, M; Henrique, R; Jeronimo, C; Moreira, N; Bastos, MD; de Pinho, PG. 2014. Analysis of volatile human urinary metabolome by solid-phase microextraction in combination with gas chromatography-mass spectrometry for biomarker discovery: Application in a pilot study to discriminate patients with renal cell carcinoma. EUROPEAN JOURNAL OF CANCER, 50, DOI: 10.1016/j.ejca.2014.04.011
  • Monteiro, MS; Carvalho, M; Bastos, MD; de Pinho, PG. 2014. Biomarkers in renal cell carcinoma: a metabolomics approach. Metabolomics, 10, DOI: 10.1007/s11306-014-0659-5
  • Silva, A; Campos, S; Monteiro, J; Venancio, C; Costa, B; de Pinho, PG; Antunes, L. 2011. Performance of Anesthetic Depth Indexes in Rabbits under Propofol Anesthesia Prediction Probabilities and Concentration-effect Relations. ANESTHESIOLOGY, 115, DOI: 10.1097/ALN.0b013e318222ac02
  • Valente, MJ; Henrique, R; Costa, VL; Jerónimo, C; Carvalho, F; Bastos, ML; de Pinho, PG; Carvalho, M. 2011. A rapid and simple procedure for the establishment of human normal and cancer renal primary cell cultures from surgical specimens. PLoS One, 6(5), DOI: 10.1371/journal.pone.0019337
  • Pontes, H; de Pinho, PG; Fernandes, E; Branco, PS; Ferreira, LM; Carmo, H; Remiao, F; Carvalho, F; Bastos, ML. 2010. Metabolic interactions between ethanol and MDMA in primary cultured rat hepatocytes. TOXICOLOGY, 270, DOI: 10.1016/j.tox.2010.02.010

Main research interests

  • 1. Development of GC-MS methodologies to determine low molecular weight compounds in different biological matrices: in vitro and ex vivo cellular systems, plasma and urine.
  • 2. Studies of “legal” drugs of abuse toxicity by using in vitro and in vivo models.
  • 3. Use of Metabolomics to prostate and renal cell carcinoma Detection and Biomarker Discovery.
  • 4. Use metabolomic to dissect mechanisms of action and to find new and sensitive biomarkers of toxicity (Toxicometabolomics).


  • PhD in Biological and Medical Sciences, University of Bordeaux, France (1994)
  • "Agregação" in Toxicology, Universidade do Porto (2019)