(Bio)molecular Structure and Interactions by NMR
Our main research interests are related to the application and development of solution state nuclear magnetic resonance (NMR) spectroscopy for structural analysis and the study of intermolecular interactions in biological and chemical systems. In the context of the functional analysis of catalytic systems and the understanding of molecular recognition processes we explore: (i) protein-ligand interactions in drug discovery; (ii) solute-solvent interactions; (iii) protein structure and dynamics (iv) new techniques in NMR.
Studying intermolecular interactions in complex media
Cells are inherently crowded, under molecular crowding (MC) conditions specific molecular recognition events and interactions may occur, that could remain undetectable under diluted solutions. We have recently used NMR to study the formation of large molecular size protein complexes driven by protein-glycan interactions under MC conditions demonstrating that glycosylation can be a player in quinary structure and in the organization of the cellular environment at the molecular level (CHEM. EUR. J., 2017; 23, 13213). Another of our main research interests is the study of molecular recognition processes in alternative complex media. By combining different NMR experiments we have been studying ion-pair interactions in ionic liquids in order to understand their supramolecular structure and better explore their biochemical applications (MAGN. RESON. CHEM., 2018, 56, 127).Protein-Glycan Quinary Interactions in Crowding Environment Unveiled by NMR Spectroscopy
Structural insights into mucin glycosylation and recognition
Glycans play a key role in distinct molecular mechanisms in health and disease. In this context, the projects (IF/00780/2015 and PTDC/BIA-MIB/31028/2017) investigate the mucin-derived cancer-related glycophenotype. By employing NMR in tandem with molecular modeling and biophysical techniques, the projects exploit the structural features of mucin glycosylation (NAT. COMMUN. 2017; 5, 1959; CHEM. EUR. J. 2018; 24, 8382) and the molecular recognition of mucin-derived tumour-associated carbohydrate antigens (TACAs) (J. AM. CHEM. SOC. 2015, 137, 12438) for a structure-guided rational design of glycan-based cancer therapies.Structural Insights on Glycans for Cancer Therapies
- Evaluation of ionic liquids for CO2 capture in exhaust gases”, PETROBRAS SA., Total and Unit funding: €110,000, Eurico Cabrita (PI).
- “Advances into the Glycome: From a structural viewpoint to function in human health and disease”, IF/00780/2015, FCT-MCTES, Total and Unit funding: €50,000, Filipa Marcelo (PI).
- “Advances in MUC1 Glycan Cancer Antigens: From structure to function in the fight against cancer”, FCT-MCTES, Total funding: €238,195, Unit funding: €176,266, Filipa Marcelo (PI).
Marcelo, F; Supekar, N; Corzana, F; van der Horst, JC; Vuist, IM; Live, D; Boons, GJPH; Smith, DF; van Vliet, SJ. 2019. Identification of a secondary binding site in human macrophage galactose-type lectin by microarray studies: Implications for the molecular recognition of its ligands. JOURNAL OF BIOLOGICAL CHEMISTRY, 294, DOI: 10.1074/jbc.RA118.004957
Marcileia Zanatta; Nathalia MSimon; Francisco Pdos Santos; Marta CCorvo; Eurico JCabrita; Jairton Dupont. 2019. Correspondence on “Preorganization and Cooperation for Highly Efficient and Reversible Capture of Low-Concentration CO2 by Ionic Liquids”. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, DOI: 10.1002/anie.201712252
Silva, JM; Giuntini, S; Cerofolini, L; Geraldes, CFGC; Macedo, AL; Ravera, E; Fragai, M; Luchinat, C; Calderone, V. 2019. Non-crystallographic symmetry in proteins: Jahn-Teller-like and Butterfly-like effects?. JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY, 24, DOI: 10.1007/s00775-018-1630-0
Silva, JM; Cerofolini, L; Giuntini, S; Calderone, V; Geraldes, CFGC; Macedo, AL; Parigi, G; Fragai, M; Ravera, E; Luchinat, C. 2019. Metal centers in biomolecular solid-state NMR. JOURNAL OF STRUCTURAL BIOLOGY, 206, DOI: 10.1016/j.jsb.2018.11.013
Faustino, André F; Barbosa, Glauce M; Silva, Micael; Castanho, Miguel ARB; Da Poian, Andrea T; Cabrita, Eurico J; Santos, Nuno C; Almeida, Fabio CL; Martins, Ivo C. 2019. Fast NMR method to probe solvent accessibility and disordered regions in proteins. Scientific Reports, 9(1), DOI: 10.1038/s41598-018-37599-z