Blue Biotechnology & Biomedicine
Our research focuses on the discovery of bioactive marine natural products as lead-like agents for drug discovery and the development of biotechnological applications. In particular, small molecules with antimicrobial, antibiofilm, antifouling and anticancer activities from marine-derived actinomycete sources collected off the Atlantic Macaronesia ecoregion and Portugal mainland. Our lab uses a multidisciplinary bioprospecting approach to Blue Biotechnology and Biomedical research combining Marine Natural Products Chemistry, Biochemistry, Molecular Biology, Microbiology, Metabolomics, Genomics, Chemo and Bioinformatics, Chemical Ecology, Pharmaceutical Sciences and Material Sciences. Simultaneously, we fill the knowledge gap on the Atlantic actinomycete biodiversity and biogeography.
Chair of the Organizing Committee of the Joint XVI International Symposium on Marine Natural Products (MANAPRO) & XI European Conference on Marine Natural Products (ECMNP) 2019, Portugal.
Targeting actinomycetes from marine sediments for the discovery of drug lead-like compounds
Pioneers in the study of actinomycetes from Portuguese oceanic sediments (10-1300 m depth) as a source for the discovery of bioactive natural product scaffolds.
To date, we have built a collection of over 400 taxonomic characterized marine-derived actinomycetes. These were used to generate small molecule libraries that were screened against bacterial pathogens and cancer cell lines. In particular, the chemistry, diversity and biogeographical patterns of marine actinomycete taxa from Portugal mainland, the Madeira and Azores Archipelagos are being tackled. The diversity of chemically rich marine actinomycete groups like MAR4 and MAR1 was broadened by the recovery of novel phylotypes.
Our research highlights the importance of bioprospecting efforts at unexplored regions of the ocean in order to recover strains belonging to distinct marine groups that produce novel and interesting chemistry (Prieto-Davó et al, FRONTIERS IN MICROBIOLOGY, 2016; Bauermeister et al, ENVIRONMENTAL MICROBIOLOGY, 2019).
Bioprospecting marine natural products for therapeutic and biotechnological applications
We have performed drug-lead dereplication using MS/MS networking and structure elucidation through 1D, 2D NMR spectroscopic techniques and HR-MS spectrometry, repurposing napyradiomycins and antibiofilm agents (Bauermeister et al, FRONTIERS IN MARINE SCIENCE, 2018; Bauermeister et al, ENVIRONMENTAL MICROBIOLOGY, 2019).
Identified metabolites have been object of a multidisciplinary biotechnological R&D strategy focused the development of novel and innovative marine-derived biotechnological applications through its incorporation into polymeric matrices of different nature, such as antimicrobial and anti-biofilm wound dressings and marine antifouling paints and coatings (patent requests: PT20161000084914; PT20161000085046; PT115055).
- "Ocean bioprospecting: targeting marine actinomycetes as a source for lead-like drug discovery", FCT funding, FCT-MCTES, IF/00700/2014, Total funding: €221,300, Unit funding: €221,300, Susana P. Gaudêncio (PI).
- “Ocean Treasures: Ocean Sediments from Madeira Archipelago - a new source of innovative and bioactive compounds”, FCT-MCTES, PTDC/QUIQUI/119116/2010, Total funding: €120,994, Unit funding: €110,797, Susana P. Gaudêncio (PI).
- “Marine Bacteria as a Novel Resource for Drug Discovery”, Marie Curie Co-fund Welcome II-DFRH/WIIA/102/2011, EU 7th Framework Programme (FP7/2007–2013) under grant agreement PCOFUND-GA- 2009-246542, Total funding: €249,000, Unit funding: €249,000, Susana P. Gaudêncio (PI).
- “Marine actinomycetes proteomics to synthetic biology – targeting Madeira Archipelago biodiversity”, EU funding Instruct project PID: 1153, Total funding: €6,000, Unit funding: €6,000, Susana P. Gaudêncio (PI).
- H2020-MSCA-RISE-2016, Project ID: 734641 “NANOMED -Nanoporous and Nanostructured Materials for Medical Applications”. Total funding: €972,000, Unit funding: €171,000, Svetlana Lyubchik (PI).
- H2020-MSCA-RISE-2015, Project ID: 690968, “NANOGUARD2AR-Nanomaterials-based innovative engineering solutions to ensure sustainable safeguard to indoor air”. Total funding: € 1,386,000, Unit funding: €306,000, Svetlana Lyubchik (PI).
Bauermeister, Anelize; Pereira, Florbela; Grilo, Ines R; Godinho, Camila C; Paulino, Marisa; Almeida, Vanessa; Gobbo-Neto, Leonardo; Prieto-Davo, Alejandra; Sobral, Rita G; Lopes, Norberto P; Gaudencio, Susana P. 2019. Intra-clade metabolomic profiling of MAR4 Streptomyces from the Macaronesia Atlantic region reveals a source of anti-biofilm metabolites.. ENVIRONMENTAL MICROBIOLOGY, DOI: 10.1111/1462-2920.14529
Cruz, S; Gomes, SE; Borralho, PM; Rodrigues, CMP; Gaudencio, SP; Pereira, F. 2018. In Silico HCT116 Human Colon Cancer Cell-Based Models En Route to the Discovery of Lead-Like Anticancer Drugs. BIOMOLECULES, 8, DOI: 10.3390/biom8030056
Bauermeister, A; Velasco-Alzate, K; Dias, T; Macedo, H; Ferreira, EG; Jimenez, PC; Lotufo, TMC; Lopes, NP; Gaudencio, SP; Costa-Lotufo, LV. 2018. Metabolomic Fingerprinting of Salinispora From Atlantic Oceanic Islands. Frontiers in Microbiology, 9, DOI: 10.3389/fmicb.2018.03021
Bauermeister Anelize, Pereira Florbela, Grilo Inês, Prieto-Davo Alejandra, Sobral Rita G, Lopes Norberto P, Gaudêncio Susana P. 2018. Molecular Networking to target the isolation of novel isoprenoids derivatives from marine-derived actinomycetes. Frontiers in Marine Science, DOI: 10.3389/conf.fmars.2018.06.00160
Dias, Tiago; Gaudencio, Susana P; Pereira, Florbela. 2018. A Computer-Driven Approach to Discover Natural Product Leads for Methicillin-Resistant Staphylococcus aureus Infection Therapy.. Marine Drugs, 17(1), DOI: 10.3390/md17010016