Molecular Microbiology & Genomics

Molecular Microbiology

The MMG Group is interested in genomics and molecular microbiology applied to pathogenesis and resistance. Past achievements relate to the understanding of microbial pathogenicity and concern the role of bacterial peptidoglycan (PG) hydrolases in evading the immune response and the role of PG amidation in antibiotic resistance. Also, characterization of Salmonella virulence proteins led to findings relevant for targeting virulence. Applied research contributed to the development of a test for detection of carbapenemases (antimicrobial resistance), which is presently marketed. Using genomics, we revealed fundamental aspects related to the emergence of domesticated microorganisms. We integrated large-scale multi-omics data to understand mechanisms leading to alterations in cancer and revealed candidate genes to be used as prognostic factors and therapeutic targets.


Our objectives for the near future are to elucidate drivers and mechanisms of antimicrobial resistance and understand virulence mechanisms of intracellular pathogens. Another aim is to unveil the physiological role of PG amidation in order to develop strategies against pathogens and elucidate the role of PG hydrolases in bacterial virulence. The role of multipurpose ATPases in microbial pathogenesis and their use as targets in therapy will be also investigated. Population and functional genomics will be used to understand crucial aspects of microbial adaptation. We aim to decipher pathological conditions in human cells and explore therapeutic targets in aging, age-related disorders and cancer.

 

In 2013-2017 the MMG group published 146 WoS papers with 1465 citations, 4 books/book chapters; supervised 19 PhD and 25 MSc theses; participated in 10 international research projects (5 of which as PI) and 35 national projects (20 as PI), approximate total funding of 2.5 M€.

MMG Research Labs
Recent publications
Nilsson, RH; Taylor, AFS; Adams, RI; Baschien, C; Bengtsson-Palme, J; Cangren, P; Coleine, C; Daniel, HM; Glassman, SI; Hirooka, Y; Irinyi, L; Irsenaite, R; Martin-Sanchez, PM; Meyer, W; Oh, SY; Sampaio, JP; Seifert, KA; Sklenar, F; Stubbe, D; Suh, SO; Summerbell, R; Svantesson, S; Unterseher, M; Visagie, CM; Weiss, M; Woudenberg, JHC; Wurzbacher, C; Van den Wyngaert, S; Yilmaz, N; Yurkov, A; Koljalg, U; Abarenkov, K. 2018. Taxonomic annotation of public fungal ITS sequences from the built environment - a report from an April 10-11, 2017 workshop (Aberdeen, UK). MycoKeys, DOI: 10.3897/mycokeys.28.20887
Pontes, A; Rohl, A; MaLdonado, C; Yurkov, AM; Sampaio, JP. 2017. Cryptotrichosporon argae sp nov., Cryptotrichosporon brontae sp nov and Cryptotrichosporon steropae sp nov., isolated from forest soils. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 67, DOI: 10.1099/ijsem.0.002177
Pedras, B; Salema-Oom, M; Sa-Nogueira, I; Simoes, P; Paiva, A; Barreiros, S. 2017. Valorization of white wine grape pomace through application of subcritical water: Analysis of extraction, hydrolysis, and biological activity of the extracts obtained. JOURNAL OF SUPERCRITICAL FLUIDS, 128, DOI: 10.1016/j.supflu.2017.05.020
Mano, F; Martins, M; Sa-Nogueira, I; Barreiros, S; Borges, JP; Reis, RL; Duarte, ARC; Paiva, A. 2017. Production of Electrospun Fast-Dissolving Drug Delivery Systems with Therapeutic Eutectic Systems Encapsulated in Gelatin. AAPS PHARMSCITECH, 18, DOI: 10.1208/s12249-016-0703-z
Botelho, J; Grosso, F; Peixe, L. 2017. Characterization of the pJB12 Plasmid from Pseudomonas aeruginosa Reveals Tn6352, a Novel Putative Transposon Associated with Mobilization of the bla(VIM-2)-Harboring In58 Integron. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 61, DOI: 10.1128/AAC.02532-16