The X-ray Diffraction facility provides the screening, testing and complete data collection from X-ray diffraction of single crystals, either from protein or small molecule compounds. Data is obtained in the in-house X-ray diffractometer or through access to synchrotron macromolecular crystallography beamlines (ESRF, DIAMOND, SLS, SOLEIL, DESY, ALBA). Diffraction is performed using an X-ray diffractometer (with KAPPA four-circle goniometer), and complete data are collected according to experimental requirements. A microspectrophotometer is available and can be coupled to the goniometer. Dedicated software permits indexing, integration, scaling of data and 3D structure solution. Small-Angle X-ray Scattering (SAXS) experiments can be performed through access to synchrotron facilities. A service for Single Crystal X-ray Structure Determination of small molecules is available. The facility integrates the Portuguese Center for Integrated Structural Biology (PCISBIO), an Affiliate Center of Instruct.

 

Equipment

  • X-ray diffractometer with KAPPA four-circle goniometer (Bruker D8 Venture)
  • IμS 3.0 microfocus Mo-Kα and Cu-Kα X-ray sources (Bruker)
  • Photon 100 CMOS detector
  • Cryostream 600 (Oxford CryoSystems)
  • ISX Stage for in-situ X-ray crystallography
  • Microspectrophotometer (4DX Systems)
  • Xe chamber for crystal derivatization (Hampton Research)
  • Automated nanodrop equipment for protein crystallization (Oryx8, Douglas Instruments)

 

Responsible

Maria João Romão

PhD in Chemistry, 1989, IST-UL Habilitation in Biochemistry, 2001, FCT NOVA Full Professor, FCT NOVA

 

Staff

  • Ana Luísa Carvalho (UCIBIO Researcher)
  • Teresa Santos-Silva (FCT Investigator)
  • Sónia Barroso (Chemical Crystallography Responsible Scientist)
  • Cecília Bonifácio (Chemical Crystallography Technician)

 

Funding

  • Fundação para a Ciência e Tecnologia
  • RECI/BBB-BEP/0124/2012
  • COST
  • BioStruct/iNEXT
  • INSTRUCT

 

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