Beom Soo Kim, Chungbuk National University
High Cell Density Culture for Production of Bioplastics & Biochemicals
Host: Maria Ascensão Reis, UCIBIO - FCT NOVA
Abstract - HIGH CELL DENSITY CULTURE FOR PRODUCTION OF BIOPLASTICS AND BIOCHEMICALS. Beom Soo Kim*
Department of Chemical Engineering, Chungbuk National University, Cheongju, Chungbuk, Republic of Korea.
*Corresponding author: Beom Soo Kim, firstname.lastname@example.org
A primary goal of fermentation research is the cost-effective production of desired products using high productivity techniques. High cell density culture techniques provide advantages such as improved productivity, reduced culture volume, enhanced downstream processing, reduced wastewater, and reduced investment in equipment. Today, many cells are routinely grown to concentrations greater than 100 g/L. High cell density of microbial cells has often been achieved by fed-batch, cell recycle, and dialysis culture. Fed-batch culture is most popular to obtain high cell density and thus has often been used for industrial production of various metabolites. It is used to prevent or reduce substrate inhibition by controlling nutrient supply. Cell recycle or retention culture techniques using a membrane filter module are effective to reduce the inhibition of various metabolites by removing them continuously through filter unit. They have been employed to produce mainly extracellular products such as ethanol and lactic acid by high cell density culture. In this presentation, several examples of high cell density culture will be introduced including production of polyhydroxyalkanoates (PHA) by fed-batch culture of Ralstonia eutropha, production of nattokinase by fed-batch culture of Bacillus subtilis, and co-production of carotenoids and PHA by cell retention culture of Paracoccus sp. LL1. In addition, other research areas such as vegetable oil-based polymers and biosynthesis of nanomaterials will be briefly discussed.
Beom Soo Kim is a professor of chemical engineering at Chungbuk National University, Cheongju, S. Korea. He studied chemical engineering at Seoul National University (1988), obtained a PhD in biochemical engineering at Korea Advanced Institute of Science and Technology (KAIST) (1993), completed postdoctoral work at MIT Prof. Robert Langer's lab (1998). He started his lab at Chungbuk National University (2001) and spent sabbatical research in Dr. Ching T. Hou's lab at National Center for Agricultural Utilization Research (NCAUR), United States Department of Agriculture (USDA), Peoria, Illinois (2005). Beom Soo Kim has published over 140 papers. Beom Soo Kim served as Editor in Chief, Korean Society for Biotechnology and Bioengineering Journal and also is an Editorial Board member of several journals such as Biocatalysis and Agricultural Biotechnology, Korean Journal of Chemical Engineering, Biotechnology and Bioprocess Engineering, Polymers, and BMC Biotechnology.