Centro Singular de Investigación en Química Biolóxica e Materiais Moleculares (CIQUS) - Universidade de Santiago de Compostela
Chemical and Synthethic Biology approached form Peptides and Supramolecular Chemistry
Host: Cecília Roque
Our research group is interested in the application of supramolecular chemistry to understand and manipulate biology. [1,2] This is because the weak and non covalent forces that control the shape and the topology of biomolecules are governed by the principles described by supramolecular chemistry. This topology is ultimately responsible of bimolecular properties and by modulating the shape we can control and improve functional behaviour.
With focus in supramolecular interactions for artificial membranes vectors we have investigated the construction of amphiphilic peptide libraries for gene and protein delivery. [3-5]
With emphasis in synthetic biology towards bottom up approaches towards simple artificial cytoskeleton mimics, we have developed supramolecular 1D polymers in confined spaces.  
This work has received financial support from Spanish Agencia Estatal de Investigación (AEI) [CTQ2014-59646-R, SAF2017-89890-R], the Xunta de Galicia (ED431C 2017/25, 2016-AD031 and Centro singular de investigación de Galicia accreditation 2016–2019, ED431G/09) and the European Union (European Regional Development Fund - ERDF). M.J. received a F.P.I. fellowship from MINECO (CTQ2014-59646-R). J.M. received a Ramón y Cajal (RYC-2013-13784), an ERC Starting Investigator Grant (DYNAP- 677786) and a YIG from the Human Frontier Science Research Program (RGY0066/2017).
 A. Fuertes, J. Marisa, J. R. Granja, J. Montenegro, Chem. Commun. 2017, 53, 7861–7871.
 I. Lostalé-Seijo, J. Montenegro, Nat. Rev. Chem. 2018, 2, 258–277.
 J. M. Priegue, D. N. Crisan, J. Martínez-Costas, J. R. Granja, F. Fernandez-Trillo, J. Montenegro, Angew. Chem. Int. Ed. 2016, 55, 7492–7495.
 I. Louzao, R. G. X. A.-F. X. García-Fandiño, J. Montenegro, J. Mat. Chem. B 2017, 5, 4426–4434.
 I. Lostalé-Seijo, I. Louzao, M. Juanes, J. Montenegro, Chem. Sci. 2017, 8, 7923–7931.
 R. Booth, I. Insua, G. Bhak, J. Montenegro, Org. Biomol. Chem. 2018, 335, 813–8.
 A. Méndez-Ardoy, J. R. Granja, J. Montenegro, Nanoscale Horizons 2018, 3, 391–396.
Javier Montenegro received his master degree (2003) and PhD (2009) from the University of Santiago de Compostela, under the direction of Prof. Susana López, working in the field of synthetic retinoids. In 2005 he was a visiting scientist in the group of Prof. Steven Ley at Cambridge University (Total Synthesis). In 2007 he was a visiting scientist at the Scripps Research Institute (La Jolla) working with Prof. Reza M. Ghadiri in prebiotic chemistry and supramolecular dynamic polymers. In 2009 he moved to the University of Geneva for postdoctoral studies with Prof. Stefan Matile in biosensing, synthetic transport systems and controlled delivery. In 2012 he returned to Spain as a Juan de la Cierva fellow to work at the CIQUS associated with Prof. Juan R. Granja. He obtained a Ramon y Cajal contract at the Organic Chemistry Department and the CIQUS in the University of Santiago de Compostela. He has received several of the most prestigious international awards including: a Starting Grant from the ERC (2015), a Young Investigator Grant of the Human Frontier Science Program (2017), JSP Fellow 52th Bürgenstock Conference (2017), the Prize of Young Investigators of the Spanish Royal Society of Chemistry (2018) and the Prize of Young Research Group Leaders in Chemical Biology of the RSEQ (2019).
At the interphase between organic synthetic and supramolecular chemistry applied to biological systems and the discovery of new materials. Focused in the topological control of supramolecular assemblies for broad applications such as differential sensing, nanotechnology, controlled delivery and tubular-templated composites.
Peptide/Cas9 Nanostructures for Ribonucleoprotein Cell Membrane Transport and Gene Edition” Lostalé-Seijo, I.; Louzao, I.; Juanes, M.; Montenegro, J. Chem. Sci., 2017, 8, 7883–8466.
“Supramolecular Functional Assemblies: Dynamic Membrane Transporters and Peptide Nanotubular Composites” Fuertes, A.; Juanes, M.; Granja. R. J.; Montenegro, J. Chem. Commun., 2017, 53, 7861-7871.
“In situ” Functionalized Polymers for siRNA Delivery”, Priegue, J. M.; Crisan, D. N.; Martínez-Costas, J; Granja, J. R.; Fernandez-Trillo, F.; Montenegro, J. Angew. Chem. Int. Ed. 2016, 55, 7492-7495.
“Self-assembly of Silver Metal Clusters of Small Atomicity on Cyclic Peptide Nanotubes”, M. Cuerva, R. García-Fandiño, C. Vázquez-Vázquez, M. A. López-Quintela, Javier Montenegro*, Juan R. Granja, ACS Nano, 2015, 9, 10834-10843.
“Cellular Uptake: Lessons from Supramolecular Organic Chemistry”, G. Gasparini, E.-K. Bang, J, Montenegro and S. Matile, Chem. Commun. 2015, 51, 10389-10402 .
“Coupling of Peptides and Carbon Nanotubes” Montenegro, J.,* Vázquez-Vázquez, C., Kalinin, A., Geckeler, K. E., & Granja, J. R. Coupling of carbon and Peptide nanotubes. J. Am. Chem. Soc., 2014, 136, 2484–2491.
“Ion Channel Models Based on Self-Assembling Cyclic Peptide Nanotubes.” Montenegro, J.; Ghadiri, M. R.; Granja, J. R. Acc. Chem. Res., 2013, 46, 2955–2965.
“Dynamic Amphiphile Libraries To Screen for the “Fragrant” Delivery of siRNA into HeLa Cells and Human Primary Fibroblasts.” Gehin, C.; Montenegro, J.; Bang, E.-K.; Cajaraville, A.; Takayama, S.; Hirose, H.; Futaki, S.; Matile, S.; Riezman, H. J. Am. Chem. Soc., 2013, 135, 9295–9298.
“Recent synthetic transport systems.” Matile, S.; Vargas Jentzsch, A.; Montenegro, J.; Fin, A. Chem. Soc. Rev., 2011, 40, 2453–2474.
“Experimental evidence for the functional relevance of anion-π interactions.” Dawson, R. E.; Hennig, A.; Weimann, D. P.; Emery, D.; Ravikumar, V.; Montenegro, J.; Takeuchi, T.; Gabutti, S.; Mayor, M.; Mareda, J.; Schalley, C. A.; Matile, S. Nat. Chem., 2010, 2, 533–538.